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Novel neisseria gonorrhoeae culture medium and method for making same

A culture medium, gonococcus technology, applied in the direction of bacteria, etc., to achieve the effects of excellent performance, convenient use, simple and reasonable preparation method

Inactive Publication Date: 2008-05-14
SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Description
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  • Application Information

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Problems solved by technology

So far, there is no licensed commercial production of gonorrhea culture medium in China

Method used

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  • Novel neisseria gonorrhoeae culture medium and method for making same
  • Novel neisseria gonorrhoeae culture medium and method for making same
  • Novel neisseria gonorrhoeae culture medium and method for making same

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with tests and specific examples.

[0024] Preliminarily determine each composition and the ratio thereof of the medium of the present invention: basal medium (1.0~2.0% peptone, 0.05~0.15% cornstarch, 0.2~0.8% K 2 HPO 4 , 0.05~0.2%KH 2 PO 4 , 0.25~1.0% Nacl, agar 1~3%), 3%~10% sheep blood, antibacterial agent (0.003~0.005% vancomycin, 0.0075~0.015% polymyxin, 0.417~0.667% nystatin , 0.005-0.01% trimethoprim).

[0025] Preparation of medium: 1. Measure 852ml of distilled water with a clean 1000ml graduated cylinder and add it to a 2000ml beaker; 2. Use an electronic balance to weigh 15 grams of peptone, 1 gram of cornstarch, 4 grams of dipotassium hydrogen phosphate (K2HPO4), phosphate Potassium dihydrogen (KH2PO4) 1 g, sodium chloride (Nacl) 5 g, agar 10 g; 3. Add peptone, cornstarch, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and sodium chloride into distilled water in turn, h...

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Abstract

The invention discloses a new type of Neisseria gonorrhoeae medium, which is characterized in that the components include basal medium, sheep blood and bacteriostatic agent: ①Basic medium: 1.0-2.0 parts of peptone, 0.05-0.15 parts of cornstarch, K2HPO40. 2-0.8 parts, KH2PO4 0.05-0.2 parts, NaCl 0.25-1.0 parts, agar 1-3 parts; ② sheep blood 3-10 parts; ③ bacteriostatic agent: vancomycin 0.003-0.005 parts or lincomycin 0.003-0.01 parts, polymyxin 0.0075-0.015 parts, nystatin 0.417-0.667 parts and trimethoprim 0.005-0.01 parts. The culture medium is suitable for grassroots medical institutions, has excellent performance, and has a simple and reasonable preparation method, which not only overcomes the shortcomings in the prior art, but also ensures the effect of separation and culture.

Description

technical field [0001] The invention relates to the technical field of in vitro biological diagnostic reagents, in particular to a novel gonorrhoeae culture medium and a preparation method thereof. Background technique [0002] Gonorrhea is a purulent inflammation of the genitourinary system caused by Neisseria gonorrhoeae, and ranks second in the incidence of all infectious diseases after influenza. Because of its own harmfulness and its synergistic effect with AIDS, it seriously threatens the healthy life of our people. [0003] Gonorrhea culture is the "gold standard" recommended by WHO for the diagnosis of gonorrhea. One of the keys to the quality of its culture is the performance of the Neisseria gonorrhoeae medium. At present, the culture media used in China are mainly imported Neisseria gonorrhoeae culture medium such as T-M medium, imported subpackaged Neisseria gonorrhoeae culture medium, domestic Guimin culture medium and self-made chocolate culture medium, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 雍刚
Owner SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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