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Novel neisseria gonorrhoeae culture medium and method for making same

A culture medium and technology of Neisseria gonorrhoeae, applied in the field of Neisseria gonorrhoeae culture medium and its preparation, to achieve the effect of ensuring the effect of isolation and culture, simple and reasonable preparation method, and excellent performance

Inactive Publication Date: 2010-05-19
SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no licensed commercial production of gonorrhea culture medium in China

Method used

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  • Novel neisseria gonorrhoeae culture medium and method for making same
  • Novel neisseria gonorrhoeae culture medium and method for making same
  • Novel neisseria gonorrhoeae culture medium and method for making same

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with tests and specific examples.

[0024] Preliminarily determine each composition and the ratio thereof of the medium of the present invention: basal medium (1.0~2.0% peptone, 0.05~0.15% cornstarch, 0.2~0.8% K 2 HPO 4 , 0.05~0.2%KH 2 PO 4 , 0.25~1.0% Nacl, agar 1~3%), 3%~10% sheep blood, antibacterial agent (0.003~0.005% vancomycin, 0.0075~0.015% polymyxin, 0.417~0.667% nystatin , 0.005-0.01% trimethoprim).

[0025] Preparation of medium: 1. Measure 852ml of distilled water with a clean 1000ml graduated cylinder and add it to a 2000ml beaker; 2. Use an electronic balance to weigh 15 grams of peptone, 1 gram of cornstarch, 4 grams of dipotassium hydrogen phosphate (K2HPO4), phosphate Potassium dihydrogen (KH2PO4) 1 g, sodium chloride (NaCl) 5 g, agar 10 g; 3. Add peptone, corn starch, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and sodium chloride into distilled water in turn, ...

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Abstract

The invention discloses a novel neisseria gonorrhoeae culture medium which is characterized in that: the invention comprises a basic culture medium, sheep blood and bacteriostatic agent: (1) the basicculture medium comprises 1.0 to 2.0 parts of peptone, 0.05 to 0.15 part of corn starch, 0.2 to 0.8 part of K2HPO4, 0.05 to 0.2 part of KH2PO4, 0.25 to 1.0 part of NaCl and 1 to 3 parts of agar; (2) 3to 10 parts of the sheep blood; (3) the bacteriostatic agent: 0.003 to 0.005 part of vancomycin or 0.003 to 0.01 part of lincomycin, 0.0075 to 0.015 part of polymyxin, 0.417 to 0.667 part of nystatinand 0.005 to 0.01 part of trimethoprim. The culture medium, which is suitable for basic-level medical organizations, has good performance, simple and reasonable preparation method, not only overcomesthe shortcomings of the existing technique, but also ensures the separating and culturing effect.

Description

technical field [0001] The invention relates to the technical field of in vitro biological diagnostic reagents, in particular to a gonorrhea culture medium and a preparation method thereof. Background technique [0002] Gonorrhea is a purulent inflammation of the genitourinary system caused by Neisseria gonorrhoeae, and ranks second in the incidence of all infectious diseases after influenza. Because of its own harmfulness and its synergistic effect with AIDS, it seriously threatens the healthy life of our people. [0003] Gonorrhea culture is the "gold standard" recommended by WHO for the diagnosis of gonorrhea. One of the keys to the quality of its culture is the performance of the Neisseria gonorrhoeae medium. At present, the culture media used in China are mainly imported Neisseria gonorrhoeae culture medium such as T-M medium, imported subpackaged Neisseria gonorrhoeae culture medium, domestic Guimin culture medium and self-made chocolate culture medium, etc. It is g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 雍刚
Owner SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
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