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Application of Walker-256 cell strain in bone tumor pain model establishment

A technology of 1. walker-256 and cell lines, applied in the fields of microorganisms, biochemical equipment and methods, animal husbandry, etc.

Inactive Publication Date: 2008-06-04
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The data show that it has good bone invasion ability, but the cell line is derived from primary breast cancer tissue in rats. So far, there is no report that the Walker-256 cancer cell line can be used to make a bone tumor pain model

Method used

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  • Application of Walker-256 cell strain in bone tumor pain model establishment
  • Application of Walker-256 cell strain in bone tumor pain model establishment
  • Application of Walker-256 cell strain in bone tumor pain model establishment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Materials and methods:

[0026] Tumor strain: Walker-256 rat ascites cancer cell line, provided by Zhejiang Academy of Medical Sciences;

[0027] Animals: SD female rats, clean grade, weighing 140-160 grams, provided by the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine;

[0028] Main instruments: XZC-2B self-control temperature hot plate instrument (produced by Shandong Academy of Medical Sciences), XZC-A pressure pain measuring instrument (produced by Shandong Academy of Medical Sciences), high-frequency mammography machine (produced in Italy); modeling method:

[0029] Model group: After the rats were anesthetized with 3% pentobarbital sodium, the abdomen was turned upward, the hair of the left hind limb was sheared, the skin was disinfected, and a small incision of about 1 cm was cut in the skin on the upper part of the tibia, and the tibia was carefully exposed, and punctured with a 5ml syringe needle first Drill a hole, then ...

Embodiment 2

[0061] 1. Materials and methods:

[0062] Tumor strain: Walker-256 rat ascites cancer cell line, provided by Zhejiang Academy of Medical Sciences; Animal: SD female rat, clean grade, weighing 150-180 grams, provided by the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine;

[0063] Main instruments: XZC-2B self-control temperature hot plate instrument (produced by Shandong Academy of Medical Sciences), XZC-A pressure pain measuring instrument (produced by Shandong Academy of Medical Sciences), high-frequency mammography machine (produced in Italy); modeling method:

[0064] Model group: After the rats were anesthetized with 3% pentobarbital sodium, the abdomen was turned upward, the hair of the left hind limb was sheared, the skin was disinfected, and a small incision of about 1 cm was cut in the skin on the upper part of the tibia, and the tibia was carefully exposed, and punctured with a 5ml syringe needle first Drill a hole, then use a 5μl mi...

Embodiment 3

[0096] 1. Materials and methods:

[0097] Tumor strain: Walker-256 rat ascites cancer cell line, provided by Zhejiang Academy of Medical Sciences; Animal: SD female rat, clean grade, weighing 150-180 grams, provided by the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine;

[0098] Main instruments: XZC-2B self-control temperature hot plate instrument (produced by Shandong Academy of Medical Sciences), XZC-A pressure pain measuring instrument (produced by Shandong Academy of Medical Sciences), high-frequency mammography machine (produced in Italy); modeling method:

[0099] Model group: After the rats were anesthetized with 3% pentobarbital sodium, the abdomen was turned upward, the hair of the left hind limb was sheared, the skin was disinfected, and a small incision of about 1 cm was cut in the skin on the upper part of the tibia, and the tibia was carefully exposed, and punctured with a 5ml syringe needle first Drill a hole, then use a 5μl mi...

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Abstract

The invention provides an application of Walker-256 cell strains in establishing a bone tumor pain model, in particular to an application of the Walker-256 cell strains in establishing a large rat bone tumor pain model. The application of the Walker-256 cell strains of the invention has successfully established the large rat bone tumor pain model. The establishment of the tumor pain model provides a useful tool for research of topics relating to bone tumor pain and particularly filtration of tumor pain resistant traditional Chinese medicines and compounds of the tumor pain resistant traditional Chinese medicines; moreover, the cell strains are derived from large rat primary breast carcinoma tissues, thereby the bone tumor pain model established by application of oncocyte can be regarded as a breast carcinoma transition model, and the invention has significant scientific research value and application prospect.

Description

(1) Technical field [0001] The invention relates to the production of a bone tumor pain model, in particular, the Walker-256 cell line is used to create a bone tumor pain model. (2) Background technology [0002] Bone cancer pain is the most common pain in cancer patients, and about one-third of advanced cancer patients will have bone metastasis. Pain caused by tumors seriously affects the quality of life of patients. Although chemotherapy, radiotherapy, three-step therapy, and bisphosphonates are used to treat bone cancer pain, a considerable part of cancer pain cannot be ideally controlled due to short-term efficacy and adverse drug reactions. The key to the development of new therapies lies in the correct understanding of the pathogenesis of cancer pain. However, the lack of corresponding animal models has hindered the understanding of the mechanism of cancer pain and the development of anti-cancer pain drugs. In recent years, there have been reports on the successful e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A01K67/027
Inventor 俞丽霞王泽时严继贵
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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