Egg-laying preventer and preparing method thereof for diamondback moth
A kind of inhibitor, the technology of diamondback moth, applied in the field of diamondback moth oviposition inhibitor and its preparation, to achieve the effect of reducing the number of eggs
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Embodiment 1
[0019] Dry the lantana leaves at 50-70°C to constant weight, crush them into dry powder, soak the dry lantana powder with methanol at room temperature according to the conventional method and dosage, and filter to obtain the methanol extract; decompose the methanol extract with activated carbon Depigmentation to golden yellow, filtering, removing methanol to prepare a methanol extract of lantana; soaking the methanol extract of lantana with chloroform, filtering, removing chloroform to prepare a chloroform extract of lantana; using an appropriate amount of methanol in a water bath Completely dissolve the chloroform extract at 60-70°C, and let it stand below 0°C for 24 hours. After the crystals are precipitated, filter the crystals and dry them naturally to obtain the yellow powdered total lithenene. The methods used are all conventional methods in the art.
Embodiment 2
[0021] Take 0.2g of lantana methanol extract, dissolve it with 2ml of ethanol in a water bath at 60-70°C, add 400ml of water and 2ml of Tween 20 into the methanol extract solution, and mix well to obtain preparation 1.
[0022] Take 0.2g of the chloroform extract of the methanol extract of lantana, dissolve it with 2.5ml of ethanol in a water bath at 60-70°C, add 300ml of water and 0.5ml of Tween 20 to the chloroform extract solution, and mix well to obtain Preparation 2 .
[0023] Take 0.2g of total petrene, a component of the chloroform extract, dissolve it in a water bath at 60-70°C with 2ml of ethanol-acetone mixture with a volume ratio of 2:1, add 400ml of water and 2ml of Tween 20, mixed well to obtain preparation 3.
Embodiment 3
[0025] Take 0.2g of lantana methanol extract, dissolve it with 3ml of ethanol in a water bath at 70°C, add 100ml of water and 0.3ml of Tween 20 to the methanol extract solution, and mix well to obtain preparation 4. Take 0.2g of chloroform extract of lantana methanol extract, dissolve it with 3ml of ethanol in a water bath at 70°C, add 100ml of water and 0.3ml of Tween 20 to the chloroform extract solution, and mix well to obtain preparation 5. Take 0.2 g of total petrene, a component of the chloroform extract, and dissolve it with 3 ml of ethanol-acetone mixture with a volume ratio of 2:1 in a water bath at 70 ° C. Temperature 20, mix uniformly, obtain preparation 6. Determination of the effects of preparations 4, 5 and 6 on egg laying of diamondback moth by cage method:
[0026] Plant japonica seedlings with 5 true leaves in a plastic cup, the diameter of the bottom of the plastic cup is 4cm, the diameter of the cup mouth is 6.5cm, and the height is 8cm. Spray preparations ...
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