Primary culture method for maintaining near term growth of large intestinal cancer tumour cell

Abstract

The invention pertains to the primary culture technical field of colorectal cancer tumor cells. The method includes: 1) obtaining a fresh tumor sample by operation, cleaning and cutting the pollution and necrosis areas, immersing in iodophor for disinfection, adopting a PBS cleaning liquid containing dual-antigen for cleaning; 2) cutting the tumor sample into small tissue blocks, still placing, removing the supernatant liquid, using the PBS cleaning liquid containing dual-antigen for cleaning, carrying out centrifugal separation and removing the supernatant liquid; 3) adopting collagenase digestion method or tissue block adhering wall method to carry out the tissue adhering wall and cell climbing out in a culture liquid; 4) continually culturing and carrying out purification processing inthe culture liquid to maintain the necessary number for the cell growth. The success rate of the conventional primary culture method for culturing the colorectal cancer tumor cells with good state isvery low (6 percent), however, the primary culture method of the invention can achieve more than 33 percent, thus significantly improving the success probability of short-term in vitro culture of theprimary colorectal cancer cells; furthermore, the technique is simple and easy, the cost is low, so as to provide a good optional platform for applying the experimental studies of the short-term primary colorectal cancer cells.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows culturing small colonic lymphocytes that are resistant against chemotherapy drugs better at treating metastatic breast carcinoma patients who have had their disease over time due to poor outcomes from traditional treatment strategies such as surgery or radiation therapies. By growing these tiny cell populations into larger tissue structures called organoids (organs containing multiple types of immune system components), they could be used experimentally to study how different chemicals might work together specifically towards developing effective therapeutic agents targeted toward specific molecular targets associated with certain diseases like lung cancer.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to grow isolated and pure cultured colonic carcinoma (CRC) cells during their own residence inside our body without causing any harmful side effects like those caused by other organisms such as germs or viruses.