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Animal vaccine immunopotentiator and production method thereof

A technology of small molecular peptides and sequences, applied in the field of animal vaccine immune enhancers, can solve the problems of high cost, not widely used, and unstable effects

Inactive Publication Date: 2008-09-03
ZHAOQING DAHUANONG BIOLOGIC PHARMA
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently known various immune enhancers (such as levamisole, lipopolysaccharide, liposome, bursalin and some cytokines, etc.) have not been widely used due to the high cost or unstable effect.

Method used

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  • Animal vaccine immunopotentiator and production method thereof
  • Animal vaccine immunopotentiator and production method thereof
  • Animal vaccine immunopotentiator and production method thereof

Examples

Experimental program
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Embodiment 1

[0036] According to the preference of Escherichia coli to different Gly, His, and Lys, a gene 5'-GGTCACAAA-3' capable of expressing the tripeptide was designed, and nine identical genes were connected in series. The concatenated genes were cloned into the pET32a vector plasmid by restriction enzymes (EcoR I and Sal I), transformed into Escherichia coli BL21 (DE3) host bacteria, and positive clones were screened by sequence determination. The obtained recombinant bacteria were named E.coli BL21 / pET32a-sBursin. (This bacterial strain has been sent to the General Microorganism Collection Center of China Microorganism Collection Committee on September 26, 2007, and the preservation number is CGMCC No.2188). The genetic character of the strain is still very stable after 100 generations in a suitable culture medium. Animal experiments have shown that the extract of the recombinant bacteria lysate has obvious immune enhancing effect on animal vaccines.

[0037] The specific method ...

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Abstract

The invention provides a bursatin of immunopotentiator kind which is a small peptide which is a monomer with amino acid sequence COOH-Gly-His-Lys-NH2 or a multimer with end-to-end of a plurality of the sequences. The immunopotentiator of the invention has sufficient stimulating activity to various of animal lymphocyte in vitro, and has sufficient immuno-enhancing activity to newcastle disease (ND) vaccine, H5 and H9 Avian Influenza vaccine.

Description

technical field [0001] The invention relates to an animal vaccine immune enhancer, in particular a small molecular peptide, which has a significant immune enhancer effect on some virus and bacterial vaccines. The invention also relates to a method for preparing the small molecule peptide. Background technique [0002] At present, animal diseases in our country are relatively complicated. The original diseases have not been effectively controlled, and new epidemics are coming one after another. It is hard to prevent and bring huge economic losses to the breeding industry. [0003] Immunopotentiators can significantly improve the function of the body's immune system, enhance the immune effect of vaccines, and reduce the occurrence of diseases, so they have become a hot topic of research in recent years. [0004] Immune enhancers can be roughly divided into the following categories according to their sources: ① Microorganisms, such as lactic acid bacteria, small coryneform bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/083C07K7/06C07K7/08C07K14/00C12N15/11C12N15/63C12N1/21A61P37/04C12R1/19
Inventor 丁家波
Owner ZHAOQING DAHUANONG BIOLOGIC PHARMA
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