Cell culture medium

A medium and cell technology, applied in cell culture medium, culture process, tissue culture, etc., can solve problems such as the reduction of lactate yield

Active Publication Date: 2008-11-19
SARTORIUS STEDIM CELLCA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One copy of the lactate dehydrogenase gene is inactivated, so cell-specific lactate production is reduced by 50%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0190] Example 1 : Batch experiments using substances according to the invention

[0191] The following final concentrations of the substances according to the invention are set after inoculation in a culture medium:

[0192] The final concentration of sodium succinate in the medium was 1 g / l

[0193] The final concentration of malic acid in the medium was 1 g / l

[0194] The final concentration of alpha-ketoglutarate in the medium was 1 g / l

[0195] The final concentration of sodium fumarate in the medium was 1 g / l

[0196] The final concentration of tartaric acid in the medium is 0.5 g / l

[0197] The final concentration of adipic acid in the medium is 0.5 g / l

[0198] The final concentration of sodium lactate in the medium is 1 g / l

[0199] The final concentration of ornithine in the medium was 1 g / l

[0200] The final concentration of citrulline in the medium was 1 g / l

[0201] The final concentration of sodium pyruvate in the medium is 0.5 g / l

[0202] These subst...

Embodiment 2

[0205] Example 2: Fed-batch experiments using substances according to the invention

[0206] The following final concentrations of the substances according to the invention are set after inoculation in a culture medium:

[0207] The final concentration of sodium succinate in the medium was 1 g / l

[0208] The final concentration of malic acid in the medium was 1 g / l

[0209] The final concentration of alpha-ketoglutarate in the medium was 1 g / l

[0210] The final concentration of sodium fumarate in the medium was 1 g / l

[0211] The final concentration of tartaric acid in the medium is 0.5 g / l

[0212] The final concentration of adipic acid in the medium is 0.5 g / l

[0213] The final concentration of sodium lactate in the medium is 1 g / l

[0214] The final concentration of ornithine in the medium was 1 g / l

[0215] The final concentration of sodium pyruvate in the medium is 0.5 g / l

[0216] As a difference from Example 1, the substances according to the invention were t...

Embodiment 3

[0223] Example 3: Fed-batch experiments with substances according to the invention and without glutamine in all media used

[0224] The following final concentrations of the substances according to the invention are set after inoculation in a culture medium:

[0225] Control, no additives

[0226] The final concentration of sodium succinate in the medium was 1 g / l

[0227] The final concentration of alpha-ketoglutarate in the medium was 1 g / l

[0228] The final concentration of sodium lactate in the medium is 1 g / l

[0229] These substances according to the present invention were detected under feeding conditions. This medium (serum- and protein-free CHO medium from the company Sigma, CR 1020, Lot: 64K2403) contains glucose at a concentration of 4.7 g / l and does not contain glutamine. The nutrient medium used for this was obtained from HyQ Corporation (R05.2 Appendix, catalog number: SH30584.04, Lot Nr: APF21457G). This nutrient medium does not contain glutamine (Table ...

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Abstract

The invention relates to nutrient media, particularly cell culture media, containing at least one substance selected among the group that comprises citric acid, succinic acid, malic acid, a-ketoglutaric acid, fumaric acid, oxaloacetic acid, isocitric acid, oxalosuccinic acid, tartaric acid, lactic acid, adipic acid, mixtures thereof, and salts, derivatives, or complexes of said acids. The invention also relates to the use of and methods for producing such cell culture media, methods for cultivating a cell culture in an inventive cell culture medium, and cells that can be obtained with the aid of such methods.

Description

technical field [0001] The present invention relates to a culture medium, in particular a cell culture medium, for culturing at least one cell and / or for harvesting polypeptides, proteins and / or vaccines from organisms, in particular from cells or cell cultures , wherein these organisms are cultured in the culture medium according to the present invention, and the above-mentioned desired polypeptide, protein or vaccine is harvested from the organisms and / or the culture supernatant. [0002] It was found in a surprising manner that from the group of citric acid, succinic acid, malic acid, alpha ketoglutaric acid, fumaric acid, oxaloacetic acid, isocitric acid, oxalosuccinic acid, tartaric acid, adipic acid, lactic acid Partially selected organic acids or salts, derivatives or complexes of organic acids are added to a medium to increase the growth rate and / or productivity of cells and / or reduce the generation of toxic metabolites. When these substances according to the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N2500/34C12N2500/30C12N2500/32C12N5/0018C12N5/00
Inventor 阿齐兹·恰伊勒
Owner SARTORIUS STEDIM CELLCA GMBH
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