Preparation method of novel subunit vaccine of pig pseudorabies

A subunit vaccine, porcine pseudorabies technology, applied in the direction of antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc. problems such as large side effects, to achieve the effect of optimizing the capsule extraction technology, solving the poor immunogenicity and simplifying the production process
CN101332297AInactive Publication Date: 2008-12-31ι™ˆε°‘θŽΊ
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ι™ˆε°‘θŽΊ
Publication Date
2008-12-31
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a manufacturing method of a novel subunit vaccine of pseudorabies in pigs. The invention is characterized in that the manufacturing method is that: the PRV-FB strain cell virus is differentially centrifugated, the virus is then suspended in the PBS again, the Mega-10 lytic virus envelope protein is added after the protein concentration of the viral antigen is regulated, the envelope protein is extracted by a centrifugation method and a moderate amount of ISCOM stroma is added, finally, the PRV novel subunit vaccine can be made after bagging and dialyzing. The invention takes the low virulent strain (V-FB strain) of the pseudorabies as the basic virus to extract the PRV-FB envelope protein which is used as the immunogen; by applying a novel Immunostimulating Complexes (ISCOM) vaccine-producing technology, the safe and effective novel subunit vaccine of pseudorabies in pigs which can be used for preventing and controlling the pseudorabies in pigs is developed.
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Description

technical field

[0001] The invention relates to a preparation method of a novel subunit vaccine of porcine pseudorabies. Background technique

[0002] Pseudorabies is a common infectious disease of many animals. Pigs are their natural hosts. After the infected pigs recover, they are recessively infected. It mainly causes abortion, stillbirth or mummification in pregnant sows, and neurological symptoms, paralysis, exhaustion and even death in newborn piglets. The mortality rate is almost 100%, the incidence rate of weaned piglets is 20%-40%, and the mortality rate is 10%-20%. Because most of the adult pigs are subclinical infections, and they become potential sources of infection due to long-term infection and detoxification, which brings great difficulties to the control and elimination of the disease. At present, the effective prevention of PR in all countries in the world is mainly vaccine immunization. The existing porcine pseudorabies vaccines include oil emulsion inact...

Claims

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