Preparation method of novel subunit vaccine of pig pseudorabies

Abstract

The invention discloses a manufacturing method of a novel subunit vaccine of pseudorabies in pigs. The invention is characterized in that the manufacturing method is that: the PRV-FB strain cell virus is differentially centrifugated, the virus is then suspended in the PBS again, the Mega-10 lytic virus envelope protein is added after the protein concentration of the viral antigen is regulated, the envelope protein is extracted by a centrifugation method and a moderate amount of ISCOM stroma is added, finally, the PRV novel subunit vaccine can be made after bagging and dialyzing. The invention takes the low virulent strain (V-FB strain) of the pseudorabies as the basic virus to extract the PRV-FB envelope protein which is used as the immunogen; by applying a novel Immunostimulating Complexes (ISCOM) vaccine-producing technology, the safe and effective novel subunit vaccine of pseudorabies in pigs which can be used for preventing and controlling the pseudorabies in pigs is developed.

Description

technical field [0001] The invention relates to a preparation method of a novel subunit vaccine of porcine pseudorabies. Background technique [0002] Pseudorabies is a common infectious disease of many animals. Pigs are their natural hosts. After the infected pigs recover, they are recessively infected. It mainly causes abortion, stillbirth or mummification in pregnant sows, and neurological symptoms, paralysis, exhaustion and even death in newborn piglets. The mortality rate is almost 100%, the incidence rate of weaned piglets is 20%-40%, and the mortality rate is 10%-20%. Because most of the adult pigs are subclinical infections, and they become potential sources of infection due to long-term infection and detoxification, which brings great difficulties to the control and elimination of the disease. At present, the effective prevention of PR in all countries in the world is mainly vaccine immunization. The existing porcine pseudorabies vaccines include oil emulsion inact...

AI Technical Summary

Problems solved by technology

Because most of the adult pigs are latently infected, and become a potential source of infection due to long-term virus-carrying and shedding, it brings great difficulties to the control and elimination of the disease

At present, the effective prevention of PR in all countries in the world is mainly vaccine immunization. The existing porcine pseudorabies vaccines include oil emulsion inactivated vaccines, traditional attenuated vaccines and gene deletion vaccines. The shortcomings of these vaccines are: such as oil emulsion inactivated vaccines. The safety of the vaccine is good, but the immune effect is not ideal, the local side effects of the injection are large, and serological tests cannot distinguish the vaccine immune antibody from the virulent antibody; the attenuated vaccine has a good immune effect, but it cannot distinguish the vaccine immune antibody from the virulent antibody, and there is virulence The risk of returning to strong; the gene deletion attenuated vaccine has a good immune effect and can distinguish vaccine immune antibodies from virulent antibodies, but gene deletion vaccines may become virulent strains due to genetic recombination and other potential unsafe issues

Therefore, improving the existing vaccines has become a difficult problem for researchers in various countries. The research on the porcine pseudorabies subunit ISCOM vaccine has been carried out abroad. The storage period is not clear, which limits the large-scale production and clinical application of vaccines

Images