Preparation process of ecological microbial agent

A microbial agent and preparation technology, which is applied in the field of microbial preparation, can solve the problems that microorganisms cannot be cultivated, information on microbial life characteristics and ecological functions cannot be obtained, and microbial resources are buried, so as to achieve favorable absorption, high practicability, and optimization The effect of extraction techniques

Inactive Publication Date: 2020-11-03
浙江九田环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most microorganisms are unculturable at this stage;
[0006] Therefore, simple extraction and plate culture counting cannot obtain information on the living characteristics and ecological functions of microorganisms in a specific ecosystem, thus burying a large number of microbial resources with great application value

Method used

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  • Preparation process of ecological microbial agent

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Experimental program
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Effect test

Embodiment 1

[0027] A preparation process of an ecological microbial inoculum, wherein the preparation method of the microbial inoculum comprises the following steps:

[0028] Step 1: Raw material processing, screening various Chinese herbal medicines, selecting Chinese herbal medicines with intact skin, and intercepting most of the herbal medicine segments;

[0029] Step 2: Prepare the bacterial flora solution carrier. There are as many as 122 types of microorganisms, which can contain anaerobic bacteria, aerobic bacteria and facultative bacteria at the same time. Using the bacterial flora solution carrier allows these 122 kinds of bacteria to coexist in the same environment , wherein the solution of the specific solution carrier for bacterial culture mainly comes from the cell fluid of fresh Chinese herbal medicines, mainly extracting the Chinese herbal medicine cell fluids in wormwood, dandelion and Houttuynia cordata plants; then take an appropriate amount of camphor wood powder, bamboo...

Embodiment 2

[0038] A preparation process of an ecological microbial inoculum, wherein the preparation method of the microbial inoculum comprises the following steps:

[0039] Step 1: Raw material processing, screening various Chinese herbal medicines, selecting Chinese herbal medicines with intact skin, and intercepting most of the herbal medicine segments;

[0040] Step 2: Prepare the bacterial flora solution carrier. There are as many as 122 types of microorganisms, which can contain anaerobic bacteria, aerobic bacteria and facultative bacteria at the same time. Using the bacterial flora solution carrier allows these 122 kinds of bacteria to coexist in the same environment , wherein the solution of the specific solution carrier for bacterial culture mainly comes from the cell fluid of fresh Chinese herbal medicines, mainly extracting the Chinese herbal medicine cell fluids in wormwood, dandelion and Houttuynia cordata plants; then take an appropriate amount of camphor wood powder, bamboo...

Embodiment 3

[0049] A preparation process of an ecological microbial inoculum, wherein the preparation method of the microbial inoculum comprises the following steps:

[0050] Step 1: Raw material processing, screening various Chinese herbal medicines, selecting Chinese herbal medicines with intact skin, and intercepting most of the herbal medicine segments;

[0051] Step 2: Prepare the bacterial flora solution carrier. There are as many as 122 types of microorganisms, which can contain anaerobic bacteria, aerobic bacteria and facultative bacteria at the same time. Using the bacterial flora solution carrier allows these 122 kinds of bacteria to coexist in the same environment , wherein the solution of the specific solution carrier for bacterial culture mainly comes from the cell fluid of fresh Chinese herbal medicines, mainly extracting the Chinese herbal medicine cell fluids in wormwood, dandelion and Houttuynia cordata plants; then take an appropriate amount of camphor wood powder, bamboo...

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Abstract

The invention discloses a preparation process of an ecological microbial agent, and particularly relates to the field of microbial preparation. A preparation method comprises the following steps: step1, treating raw materials; step 2, preparing a bacterial flora solution carrier; step 3, performing DNA analysis; step 4, performing in-situ cell lysis; step 5, performing DNA purification. The wholeprocess are reasonable in steps, a Chinese herbal medicine extraction technology and a Chinese herbal medicine bacterium extraction technology based on a DNA extraction technology are optimized, theproduct obtained through the DNA extraction technology contains up to 122 kinds of microorganisms, the combined action of multiple microorganisms is more beneficial to absorption and decomposition ofharmful gas, and besides, the microorganisms can also generate inorganic acid. Besides, an acid environment unfavorable for the life of spoilage microorganisms is formed, substances generating malodorous gas are fundamentally degraded and decomposed, and specific microorganisms can be screened, domesticated and cultured through a self-owned technology according to different components of the malodorous gas, so that the product is more targeted and has higher practicability.

Description

technical field [0001] The invention belongs to the technical field of microorganism preparation, and in particular relates to a preparation process of an ecological microbial bacterial agent. Background technique [0002] Compared with traditional microbial extraction techniques, microbial community diversity and structure analysis is to isolate and culture microorganisms, and then analyze them through general biochemical traits and specific phenotypes. Traditional extraction techniques have the following limitations: [0003] limited to the isolation of microorganisms from solid media; [0004] Most of the pure culture methods and principles are introduced from medical microorganisms. Some methods are not very suitable for the research of specific microorganisms. The total number of bacteria, a large number of oligotrophic microorganisms are not suitable for growth, and the error of the measurement results is large; [0005] Most microorganisms are unculturable at this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/689C12Q1/6806
CPCC12N1/20C12Q1/6806C12Q1/689
Inventor 张雅萍
Owner 浙江九田环保科技有限公司
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