Kit for broad spectrum cancer hybridization in situ, detection method and application thereof

A detection kit and in situ hybridization technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of unreported TROP-2 gene detection technology, imperfect design ideas of anticancer drugs, tumor cell Drug resistance and other problems, to achieve the effect of radically curing malignant diseases, high sensitivity and strong specificity

Inactive Publication Date: 2009-02-11
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003]In 2005, the American Institute of Health, the Cancer Institute, the Centers for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer" , that is to say, the cancer mortality rate has not been reduced, and it lists several factors that lead to the failure of the anti-cancer war: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete design of anticancer drugs, etc.
However, there is no report about the in situ hybridization detection kit and detection technology of TROP-2 gene

Method used

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  • Kit for broad spectrum cancer hybridization in situ, detection method and application thereof
  • Kit for broad spectrum cancer hybridization in situ, detection method and application thereof
  • Kit for broad spectrum cancer hybridization in situ, detection method and application thereof

Examples

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Effect test

Embodiment 1

[0044] A broad-spectrum cancer in situ hybridization detection kit, including a hybridization probe, a marker, and a potentiator, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0045] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0048] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0051] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0052] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0...

Embodiment 2

[0095] A kind of TROP-2 gene in situ hybridization detection method and kit application

[0096] 1. Specimen processing

[0097] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0098] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0099] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0100] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condition...

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Abstract

The invention relates to a kit for detecting in situ hybridization of broad-spectrum cancer, comprising a hybridization probe and a label, and the sequence of the hybridization probe is shown in SEQ ID NO.1. The invention also provides a method for detecting in situ hybridization of TROP-2 gene. In addition, the invention provides the application of the kit in the preparation of medicine for detecting cancers and diseases, and the cancers includes lung cancer, gastric cancer, breast cancer, carcinoma of colon, prostatic cancer, uterine cancer, cancer of pancreas, carcinoma of urinary bladder, pituitary cancer or glioma. The kit provided by the invention has the characteristics of high sensitivity and better particularity. The detecting method of the invention is convenient and simple in operation, and can be generally used and popularized in the hospitals which are above county level.

Description

【Technical field】 [0001] The invention relates to a kit, in particular to a broad-spectrum cancer in situ hybridization detection kit and its detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.7 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. In the world, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner NATUREGEN BIOTECH SHANGHAI
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