Isolated culture plant strain regeneration method for ripening seed of Bahiagrass

A technology of in vitro culture and bahia grass, applied in the field of plant tissue culture, can solve the problem of difficult to obtain granular embryogenic callus, and achieve the effect of maintaining plant regeneration ability

Inactive Publication Date: 2011-05-11
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, plant regeneration in vitro has not been reported on Bahia chinensis. The main reason is that it is difficult to obtain granular embryogenic callus

Method used

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  • Isolated culture plant strain regeneration method for ripening seed of Bahiagrass
  • Isolated culture plant strain regeneration method for ripening seed of Bahiagrass
  • Isolated culture plant strain regeneration method for ripening seed of Bahiagrass

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Experimental program
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Embodiment Construction

[0025] 1. Materials and Methods

[0026] 1.1 Test material

[0027] The variety "Sunflower" of Bahia grass was used as the test material, and its mature seeds were used as explants. The test material was provided by Beijing Forestry University Science and Technology Co., Ltd.

[0028] 1.2 Medium

[0029] The basic medium consists of MS macroelements, MS trace elements (Murashige T, Skoog F.A revised medium from rapid growth and bioassays with tobacco tissue cultures[J]. Physiol plant, 1962, 15:473~497) and B 5 Vitamin (Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells [J]. Exp. Cell Res., 1968.50: 151-158), a commonly used medium in plant tissue culture;

[0030] Callus induction medium and callus subculture medium additional hormone: 2,4-dichlorophenoxyacetic acid (abbreviated as 2,4-D, Shanghai Chemical Reagent No. 4 Factory) 2.0, 3.0, 4.0 mg / L, 6 - Benzylaminopurine (abbreviated as BAP, Shanghai Xueman Biotechnology Co., L...

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Abstract

The invention relates to a plant regeneration method for isolated culture of a mature seed of paspalum notatum, which belongs to a plant tissue culture method. The method takes the mature seed of the paspalum notatum as an explant material; the inductivity of a white, wet and particular callus reaches 50 percent on a callus induction medium of auxiliary hormones - 2.0 milligrams per liter of 2, 4-dichlorphenoxyacetic acid and 0.5 milligram per liter of 6-benzyl adenine phosphate; a white or light yellow, dry and particular callus maintains the embryonic state on the callus induction medium ofthe auxiliary hormones -2.0 milligrams per liter 2, 4-dichlorphenoxyacetic acid and 0.5 milligram per liter of the 6-benzyl adenine phosphate; and after the particular callus after secondary culture for four generations continuously is transferred onto a callus histo-differentiation medium of auxiliary hormones-2.0 milligrams per liter of N-(2-Chloro-4-pyridinyl)-N'-phenylurea and 0.05 milligram per liter of rhodofix, the differentiation rate of green planets reaches more than 30 percent. The technology is suitable for micropropagation of the paspalum notatum, screening of somatic mutants andgenetic transformation.

Description

technical field [0001] The invention relates to a plant regeneration method for in vitro culture of bahia grass mature seeds, which relates to a culture technology for acceptor materials suitable for micropropagation, screening of somatic cell mutants and gene transformation, and belongs to plant tissue culture methods. Background technique [0002] Bahia grass (Paspalum notatum Flugge.) is a plant of the genus Paspalum in the Gramineae family. It is native to Latin America. Its distribution range is between 30° north latitude and 30° south latitude and the vast tropical and subtropical regions with an altitude of <2000m. It is widely introduced and cultivated in North America, Latin America, Australia, Africa, Japan and my country. The application range of bahia grass in Taiwan Province of my country has been developed from the form of fruit (mulberry, tea) orchard coverage, step wall grass planting, mountain side ditch grass planting, slope grass belt and other forms to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 叶晓青郑安俭佘建明汤日圣王松凤
Owner JIANGSU ACAD OF AGRI SCI
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