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Breeding method for cultivated silkworm breed variety of fluorine resistant larva and non-palea perfect insect wing

A technology of fluoride resistance and adult insects, which is applied in animal husbandry and other fields to achieve the effect of heavy workload, poor selection reliability and long breeding cycle

Inactive Publication Date: 2009-05-13
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

[0006] Technical problem: The purpose of this invention is to solve the technical problem of using molecular markers in silkworm assisted breeding selection, and to provide a breeding method for silkworm varieties that use molecular marker assisted selection to select fluorine-resistant larvae and adult wingless silkworm species

Method used

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Embodiment Construction

[0020] 1. Description of the materials used

[0021] Existing research results have shown that the adult silkworm wing scaleless trait is controlled by the recessive single gene nlw, and the adult wing scaleless trait can only be expressed when the gene is homozygous (Zhang Zhifang, Qin Jian. Studies on transparent wings of silkworm moths. Silkworm Industrial Science, 1991, 17(3): 186-187.); the tolerance of silkworm to fluoride is controlled by the dominant dominant gene Def (the fluoride resistance threshold is 200mg / kg) (Lin Changlin, Mi Yidian, Yao Qin, etc. The discovery of the main effect gene of fluoride resistance in silkworm, Sericulture Science, 1997, 23 (4): 237-239), as long as the gene exists, no matter it is heterozygous or homozygous, it all shows that it is resistant to fluoride. tolerance.

[0022] (1), parent U13 of the silkworm's scaleless mutant (publicly known and public, Zhang Zhifang, Qin Jian. Research on transparent wings of silkworm moths. Sericultur...

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Abstract

The invention discloses a breeding method of solkworn strain wherein the lavae is fluoride-resistant and the imago fin has no palea scale. The breeding method uses the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' as recurrent parents and the T6 is used as the fluoride-resistant high-grade object character donor parent and the gene mutation strain U13 is used as the no palea scale gene donor. The first hybridization generation obtained by hybridizing the recurrent parents with theU13 is hybridized with the T6 and then the obtained filial generation is continuously backcrossed with the recurrent parents and each generation is subjected to individual selection during breeding and the selected individual is marked by molecule to select the genotype thereof two silkworn strains 'JsFnlw' and 'HyFnlw' are selected after self-cross homozygosis for six generations, wherein the lavae is fluoride-resistant and the imago fin has no palea scale. The 'JsFnlw' and 'HyFnlw' have strong physical constitution and the lavae whole-instars fluoride-resistance rate is above 100mg / kg and the fluoride-resistance performance is higher than the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' by 50% and the imago fin has no palea scale, in addition the micro-dust produced during seed-production is reduced by 50% than the common silkworn strain and the practical character corresponds with the utility breed level.

Description

technical field [0001] The invention relates to a breeding method for larvae resistant to fluorine and adult wingless silkworm varieties, more specifically, to a molecular marker-assisted selection method for breeding larvae fluorine-resistant and adult wingless silkworm varieties method. technical background [0002] Sericulture and silk reeling have a history of more than 5,000 years in my country. It is one of the most distinctive traditional advantageous industries in my country and one of the few products that can dominate the international market. At present, my country's silkworm cocoon and raw silk production ranks first in the world First, they respectively account for about 75% of the world's total output. [0003] The silk industry not only plays an important role in increasing farmers’ income and expanding labor employment, but also the food of silkworms—mulberry trees also play an important role and significance in environmental protection and returning farmland...

Claims

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Application Information

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IPC IPC(8): A01K67/04
Inventor 徐安英李木旺侯成香孙平江张月华钱荷英郭锡杰
Owner JIANGSU UNIV OF SCI & TECH
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