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A kind of breeding method of silkworm variety with fluorine-resistant larvae and adult wingless hairs

A fluorine-resistant and larvae-resistant technology, which is applied in animal husbandry and other directions to achieve the effects of large workload, long breeding cycle and poor selection reliability.

Inactive Publication Date: 2011-12-07
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

[0006] Technical problem: The purpose of this invention is to solve the technical problem of using molecular markers in silkworm assisted breeding selection, and to provide a breeding method for silkworm varieties that use molecular marker assisted selection to select fluorine-resistant larvae and adult wingless silkworm species

Method used

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Embodiment Construction

[0020] 1. Description of the materials used

[0021] Existing research results have shown that the adult silkworm wing scaleless trait is controlled by the recessive single gene nlw, and the adult wing scaleless trait can only be expressed when the gene is homozygous (Zhang Zhifang, Qin Jian. Studies on transparent wings of silkworm moths. Silkworm Industrial Science, 1991, 17(3): 186-187.); the tolerance of silkworm to fluoride is controlled by the dominant dominant gene Def (the fluoride resistance threshold is 200mg / kg) (Lin Changlin, Mi Yidian, Yao Qin, etc. The discovery of the main effect gene of fluoride resistance in silkworm, Sericulture Science, 1997, 23 (4): 237-239), as long as the gene exists, no matter it is heterozygous or homozygous, it all shows that it is resistant to fluoride. tolerance.

[0022] (1), parent U13 of the silkworm's scaleless mutant (publicly known and public, Zhang Zhifang, Qin Jian. Research on transparent wings of silkworm moths. Sericultur...

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Abstract

The invention discloses a method for breeding silkworm varieties whose larvae are resistant to fluorine and whose adults have no scales. In the method, Jingsong and Haoyue are used as recurrent parents, T6 is the donor parent for high-quality target traits of fluorine resistance, and the gene mutation line U13 is non-scalable. Scale gene donor. The recurrent parent was crossed with U13 to obtain the first generation of hybridization and then crossed with T6. The offspring obtained were continuously backcrossed with the recurrent parent. Individual selection was carried out during the breeding process of each generation. After 6 generations of homozygous crosses, two silkworm varieties "JsFnlw" and "HyFnlw" with fluorine-resistant larvae and scaleless adult wings were bred. The production varieties Jingsong and Haoyue are 50% higher, and the adults have no scales on their wings. The dust generated in the seed production process is 50% lower than that of ordinary silkworm varieties, and their economic characteristics reach the level of production varieties.

Description

technical field [0001] The invention relates to a breeding method for larvae resistant to fluorine and adult wingless silkworm varieties, more specifically, to a molecular marker-assisted selection method for breeding larvae fluorine-resistant and adult wingless silkworm varieties method. technical background [0002] Sericulture and silk reeling have a history of more than 5,000 years in my country. It is one of the most distinctive traditional advantageous industries in my country and one of the few products that can dominate the international market. At present, my country's silkworm cocoon and raw silk production ranks first in the world First, they respectively account for about 75% of the world's total output. [0003] The silk industry not only plays an important role in increasing farmers' income and expanding labor employment, but also plays an important role and significance in environmental protection and returning farmland to forests. Therefore, in many areas, ec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
Inventor 徐安英李木旺侯成香孙平江张月华钱荷英郭锡杰
Owner JIANGSU UNIV OF SCI & TECH
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