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Nucleotide sequence and method for identifying radix cyathulae genunie medicinal materials

A technique of nucleotide sequence and Achyranthes sichuanensis is applied in the field of identification of traditional Chinese medicinal material germplasm resources, which can solve the problems of genetic background and few research reports on kinship, and achieve the effect of simple operation and accurate distinction.

Active Publication Date: 2009-06-03
雅安三九中药材科技产业化有限公司
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Problems solved by technology

At present, in the research on the genetic differentiation and genetic diversity of Achyranthes sichuanensis, in addition to the current main research methods such as morphology and anatomy, cytology, pollenology and phytochemistry, the genetic diversity among populations of Achyranthes sichuanensis is systematically studied at the molecular level. There are few research reports on background and genetic relationship

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  • Nucleotide sequence and method for identifying radix cyathulae genunie medicinal materials
  • Nucleotide sequence and method for identifying radix cyathulae genunie medicinal materials
  • Nucleotide sequence and method for identifying radix cyathulae genunie medicinal materials

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Embodiment

[0057] In this embodiment, the ribosomal DNA ITS sequences of 19 Achyranthes bidentata germplasm resources (including 10 copies of Achyranthes bidentata, 6 copies of Achyranthes bidentata, 1 part of hybrid Achyranthes bidentata and 2 copies of Achyranthes bidentata) from different regions were determined, using The method includes the following specific steps:

[0058] (1), total DNA extraction:

[0059] Collect fresh and healthy young leaves of Achyranthes bidentata and other samples, and use the improved cetyltrimethylammonium bromide (CTAB) method to extract total DNA. The specific method is as follows:

[0060] ① Sampling and grinding:

[0061] Weigh about 2g of young leaves of disease-free and healthy plants, cut them into pieces, put them in a mortar, pour liquid nitrogen into them for quick freezing, and quickly grind the leaves into powder;

[0062] ②Water bath warm bath:

[0063] Before thawing, transfer the leaf powder into a 1.5mL centrifuge tube, add 650μL of 2%...

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Abstract

The invention discloses a nucleotide sequence for identifying radix cyathulae genunie medicinal materials; the sequence is rDNA ITS sequence from genuine radix cyathulae (white bidentata) used for identifying radix cyathulae genunie medicinal materials; analyzing the hereditary feature of radix cyathulae germ plasm resource on DNA level supplies effective molecular marker for differentiating the genuine radix cyathulae, mixed radix cyathulae and radix achyranthis bidentatae and so on, and is good for truth identification of kinds and quality identification of the radix cyathulae genunie medicinal materials and filtering the better kinds. Furthermore, the invention provides a method of identifying radix cyathulae genunie medicinal materials using the nucleotide sequence; the method has simple and accessible operation and can make a purpose of conveniently, rapidly, objectively and exactly differentiating the radix cyathulae genunie medicinal materials with other achyranthes materials.

Description

technical field [0001] The invention belongs to the technical field of identification of germplasm resources of traditional Chinese medicinal materials, and in particular relates to a nucleotide sequence and a method for identifying authentic medicinal materials of Sichuan Achyranthes bidentata. Background technique [0002] Achyranthes achyranthes is a plant of the genus Cyathula Blume in the family Amaranthaceae, also known as sweet knee, which is a perennial herb; it is a well-known authentic Chinese medicinal material produced in Sichuan, which is very commonly used and is an important export species. After 1977, various editions of the "Chinese Pharmacopoeia" determined that the plant source of Achyranthes bidentata is C. officinalis Kuan. (that is, Achyranthes bidentata). The main components of Achyranthes bidentata are alkaloids and sterols, such as cyastelone, cephalanthrone, ecdysterone, erythrosterone, etc., as well as ferulic acid; A biologically active polysacch...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/29
Inventor 田孟良徐攀辉袁继超范巧佳郑顺林唐莉刘天成王巧胡丹胡莹莹
Owner 雅安三九中药材科技产业化有限公司
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