Cell preparation for liver injury and hepatic cirrhosis and preparation thereof

A technology for cell preparations and liver damage, applied in the field of cell preparations and its preparation, to improve liver function and repair liver microvessels

Inactive Publication Date: 2012-07-11
BEIJING HEALTH & BIOTECH (H&B) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some scholars have proved that umbilical cord tissue stem cells can alleviate liver fibrosis, there is no precedent for the application of umbilical cord cell transplantation to promote liver cell regeneration while repairing liver microvessels to treat liver injury diseases and improve liver function

Method used

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Examples

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Embodiment Construction

[0027] The present invention will be further described in detail through specific examples below, and the following embodiments are only for illustration rather than limitation of the present invention.

[0028] The method of isolating cells from the umbilical cord and placenta is: mechanically cut the umbilical cord and placenta into 1mm 3 Small tissue pieces were digested with collagenase I (1mg / mL) at 37°C for 1 hour, and trypsin (0.25%) at 37°C for 30 minutes, with gentle shaking several times during the period, and fetal bovine serum (FBS) was added to neutralize the trypsin Pass the tissue suspension through a cell sieve, collect the cell suspension by centrifugation, and inoculate on a 4 μg / cm 2 Fibronectin (Fibronectin) in the T75 culture flask, cultured in the D / F12+10% fetal bovine serum (FBS) system added with 10ng / mL epidermal growth factor (EGF) for 5 to 7 days, showed fibroblast-like The cells are tissue stem cells, which are digested, passaged, purified, and ex...

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PUM

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Abstract

The invention relates to cell preparation and a preparation method thereof, and in particular discloses stem cell preparation sourced from placenta and an umbilical tissue for treating liver damage disease and a preparation method thereof. The cell preparation comprises liver cell sample cells and a PBS solution, wherein the liver cell sample cells are prepared by in vitro induction culture of the stem cells sourced from the placenta and the umbilical tissue. The preparation method comprises the following steps: firstly, separating, purifying and culturing tissue stem cells from an umbilical cord and the placenta, and preparing cell suspension; secondly, performing in vitro induction on the tissue stem cells in the first step, and differentiating the tissue stem cells into the liver cell sample cells; and thirdly, using the sterile PBS buffer solution to dilute the liver cell sample cells in the second step, and preparing the cell preparation. When the cell preparation is transplantedinto the body, the cell preparation not only can be differentiated into liver cells but also can be differentiated into endothelial cells, simultaneously secretes various growth factors, repairs liver capillaries, participates in liver cell hyperplasia, and improves the liver functions.

Description

technical field [0001] The invention relates to a cell preparation and a preparation method thereof. Specifically, the cells in the cell preparation come from stem cells of umbilical cord and placenta tissue, and can be used to treat liver tissue damage diseases after being transplanted into a human body. Background technique [0002] Liver fibrosis is a common pathological change in many liver diseases. Advanced liver fibrosis can progress to cirrhosis, portal hypertension, liver failure, and ultimately requires liver transplantation. The current treatment methods mainly include supportive treatment, symptomatic treatment and anti-fibrosis treatment. However, the disadvantage of the current anti-fibrosis treatment is that anti-fibrosis drugs are ineffective against the fibrosis caused by activated liver satellite cells, and will produce various side effects. However, recent studies have shown that even advanced liver fibrosis can be reversed, which has attracted the interes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08A61K35/50A61P1/16
Inventor 赵宝娜
Owner BEIJING HEALTH & BIOTECH (H&B) CO LTD
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