Anti-tumor DNA vaccine and preparation method thereof

A DNA vaccine and anti-tumor technology, applied in the field of medicine, can solve the problems of weak tumor targeting and DNA vaccine immunogenicity, and achieve the effect of improving immunogenicity, high safety, and strong targeting

Inactive Publication Date: 2009-07-22
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the imm

Method used

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  • Anti-tumor DNA vaccine and preparation method thereof
  • Anti-tumor DNA vaccine and preparation method thereof
  • Anti-tumor DNA vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0069] 1. Acquisition of mtHsp70 and HSV-tk genes

[0070] (1) Design primers:

[0071] According to the mRNA sequences of HSV-TK (genbank NO.J02224.) and Mycobacterium tuberculosis Hsp70 gene (genbank NO.BX842573), the specific primers for amplifying the full-length cDNA of HSV-TK and mtHsp70 genes were respectively designed by using the primer design software DNA club. The sequence is as follows:

[0072] HSV-TK primers:

[0073] Forward: 5 GCGCCTTGTAGA AGCGCG TA-3' (SEQ NO.1)

[0074] Reverse: 5' CCTTCCGGTATTGTCTCCCTTCC-3' (SEQ NO.2)

[0075] Both the upstream primer and the downstream primer introduce a restriction endonuclease Not I site (framed partial sequence);

[0076] Hsp 70 primer:

[0077] Forward: 5 CGCCTACATGGCTCGTGC-3' (SEQ NO.3)

[0078] R Reverse: 5 TCACTTGGCCTCCCGG-3' (SEQ NO.4)

[0079] Upstream and downstream primers introduce restriction endonuclease Xho I and EcoR I sites (framed partial sequences) respectively;

[0080] Submit the above-des...

example 2

[0147] 1. Acquisition of mt Hsp70 and HSV-tk genes: the same as Example 1.

[0148] 2. Construction of pCMV-mt Hsp70-IRES-tk: same as Example 1.

[0149] 3. Preparation of the DNA vaccine of the present invention: transform pCMV-mtHsp70-IRES-tk into Lactobacillus, and the specific operation is the same as in Example 1.

example 3

[0151] 1. Acquisition of mt Hsp70 and HSV-tk genes: the same as Example 1.

[0152] 2. Construction of pCMV-mt Hsp70-IRES-tk: same as Example 1.

[0153] 3. Preparation of the DNA vaccine of the present invention: transform pCMV-mt Hsp70-IRES-tk into bifidobacteria, and the specific operation is the same as in Example 1.

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Abstract

The invention provides an anti-tumor DNA vaccine, which is obtained by transforming pCMV-mt Hsp70-IRES- suicide genes into amphimicrobion which is friendly to human body, anaerobic bacteria which are friendly to the human body, amphimicrobion for reducing toxicity or obligate aerobes for reducing toxicity. The pCMV-mt Hsp70-IRES- suicide genes are recombinant plasmids which are formed by taking pCMV plasmids as an expression vector and tubercle bacillus heat shock protein 70 proteins and suicide genes as antigen genes, wherein the tubercle bacillus heat shock protein 70 proteins and the suicide genes are positioned on the upstream and the downstream of internal ribosome access sites in the pCMV plasmids respectively. The DNA vaccine is used for treating solid tumor and applied through local injection.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a medical preparation, especially a medical preparation for gene therapy. Background technique [0002] Malignant tumor is a kind of disease that seriously threatens human life and health. According to incomplete statistics, about 1.3 million patients die of cancer every year in my country, and about 7 million people in the world are killed by tumor every year. Surgery, radiotherapy, and chemotherapy can only cure about 45% of tumors, and 55% of tumor patients cannot be cured due to advanced or highly malignant tumors. The prevention and treatment of tumors has become an important mission of medical workers around the world, and all countries have invested a lot of manpower and material resources. [0003] Tumor vaccine is an important means of tumor biotherapy, and has become one of the hot spots in anti-tumor research. Tumor vaccines induce cellular immunity and humoral immune respons...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P35/00C12N15/52
Inventor 曾曙光张积仁章锦才
Owner SOUTHERN MEDICAL UNIVERSITY
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