Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Uses of DUSP21 gene

A technology of gene and gene chip, applied in the application field of DUSP21 gene, can solve problems such as unclear relationship and achieve accurate diagnosis

Inactive Publication Date: 2009-07-29
CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The relationship between DUSP21 gene and the occurrence of liver cancer is still unclear, and further research is needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uses of DUSP21 gene
  • Uses of DUSP21 gene
  • Uses of DUSP21 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] RT-PCR assay was used to detect the expression of DUSP21 gene in liver cancer tissues.

[0035] 1. Tissue isolation

[0036] The tissues used in the experiment came from 72 surgical patients with HBV-positive primary liver cancer expressing alpha-fetoprotein (RT-PCR confirmed that AFP was positive in liver cancer and negative in adjacent tumors). Once the surgically resected liver was isolated from the body, the lesion and surrounding paracancerous tissues 5 cm away were quickly excised and stored in liquid nitrogen (-80°C). The diagnosis of cancer and paracancer is based on pathological diagnosis.

[0037] 2. Total RNA extraction kit

[0038] The RNA extraction reagent uses TRIzolreagent (GIBCO / BRL), which is produced based on the one-step extraction method with acidic phenol. The utensils and water used for RNA extraction must be RNase-free to ensure an RNase-free environment in the experiment.

[0039] 3. RNA extraction steps

[0040] Dry-bake the pestle, homoge...

Embodiment 2

[0051] Embodiment 2 real-time quantitative PCR experiment

[0052] Relative quantitative method was used to detect the expression difference of DUSP21 gene in liver cancer samples (ThermalCyclerDiceTM Real Time System TP800, Takara; Premix Ex Taq™, Takara). Real-time quantitative PCR detection showed that the expression level of DUSP21 gene in liver cancer tissues was significantly higher than that in paracancerous tissues, and the t test showed P<0.01. The results of this experiment show that liver cancer can be diagnosed by real-time quantitative PCR: PCR primers for DUSP21 gene are designed to detect the RNA content of DUSP21 gene in tumor tissue. A high RNA content indicates a high possibility of liver cancer, and vice versa.

Embodiment 3

[0053] Example 3 in situ hybridization

[0054] Use DUSP21 gene probe to carry out in situ hybridization with tumor slices, the stronger the positive hybridization signal, the higher the possibility of suffering from liver cancer. The experimental steps are as follows: the liver tumor tissue is taken, embedded in OCT, quick-frozen in liquid nitrogen, and frozen in a constant temperature refrigerator for sectioning, and the frozen section is further used for in situ hybridization.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of DUSP21 gene which is used for preparing products for diagnosing liver cancer and medicines for treating the cancer. The DUSP21 gene of the invention can be used as the peculiar marker gene for diagnosing liver cancer, facilitates the diagnosis on the liver cancer to be more accurate and rapid and provides new therapeutic targets and effective medicines for preventing and treating liver cancer.

Description

technical field [0001] The present invention relates to a gene, in particular to the application of a DUSP21 gene. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage. Once symptoms appear, it is often in the middle and late stages. Therefore, the treatment is difficult and the curative effect is poor. Generally, the survival time after the onset is only 6 months. It is called "the king of cancer". Therefore, improving the level of early diagnosis of liver cancer, choosing scientific and reasonable treatment methods, and taking effective measures to prevent recurrence and metastasis will be the three key links to change this situation. [0003] At present, the most important tumor marker for the diagnosis of primar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/577A61K31/713A61K48/00A61K38/17A61K39/00A61P35/00
Inventor 邓庆宗伟英黄健张新韩泽广
Owner CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com