Clonal plant drought resistance appraisement method based on physiological integration ability

An evaluation method and drought resistance technology, applied in the field of agricultural biology, can solve problems such as poor drought resistance, and achieve the effect of strong applicability, easy promotion and objective conclusions

Inactive Publication Date: 2009-08-05
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology

For example, white clover, studies have shown that its single plant has poor drought resistance, but when multiple ramets are connected to each other through internodes, the ramets under drought stress show extremely strong drought resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The vigorous plants selected from the seedlings of 6 varieties of Bison grass were propagated by stolon cloning and branching, and a large number of (same genotype) clone populations of each variety were obtained, from which the interconnected clone sisters with the same growth state were selected. After washing away the root matrix, the ramets are kept connected and the root system of the sister plant is completely immersed in a 200ml culture container containing 30% PEG-6000 (m / v) solution, such as the sister plant is completely immersed in a 200ml culture container containing 1.0% acid fuchsin ( In the 200ml Erlenmeyer flask of m / v) solution, utilize oxygen pump to supply oxygen to root system during cultivation. After the two ramets were completely immersed in the culture solution, start counting the time, and observe the transmission of the acid fuchsin solution in the ramets every 1 hour until the leaves of the ramets under stress turned completely red or there was...

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Abstract

A clone plant drought resistance evaluation method based on the physiological integration capacity is characterized in that one plant division used as a treating plant in connected clone plant divisions is cultured in polyethylene glycol PEG-6000 solution with the weight bulk ratio being 10-30 percent to perform simulated moisture intimidation, and the other plant division used as a contrast plant is cultured in solution containing acid fuchsin, thus, by observing the transportation speed of the solution containing acid fuchsin towards the intimidated plant division direction, people can rapidly evaluate the drought resistance of the clone plate through comparing the moisture physiological integration capacity.

Description

technical field [0001] The invention relates to a method for evaluating drought resistance of cloned plants based on physiological integration ability, which belongs to the field of agricultural biotechnology. Background technique [0002] Clone plants are a group of plants that widely exist in nature, and are characterized by the ability to form many interconnected clones through the vegetative growth of roots, buds, tillers, branches, and stolons connected to the base plant. In the natural environment, the resources required for plant growth (such as light, water, and nutrients) are extremely unevenly distributed in time and space (resource heterogeneity). Cloned plants can achieve physiological integration between cloned ramets through material and energy exchange and sharing between connected ramets, effectively alleviating the adverse effects of resource heterogeneity on growth and reproduction, so that Cloned plants can adapt to harsh heterogeneous environments and pl...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 钱永强韩蕾巨关升刘俊祥孙文元赵阳孙振元
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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