Blood serum sample treatment preparation used for protein suspending chip detection

Abstract

The invention discloses a serum sample treating preparation used in a method for detecting a liquid phase protein by a suspension chip. The preparation comprises basic buffer solution, polyvinyl alcohol and polyvinylpyrrolidone, wherein the pH value of the basic buffer solution is between 5.5 and 9.0; the mass percentage of the polyvinyl alcohol in volume is between 0.05 and 1.5 percent; and the mass percentage of the polyvinylpyrrolidone in volume is between 0.05 and 2 percent. Because the serum sample treating preparation contains macromolecule surfactants of the polyvinyl alcohol and the polyvinylpyrrolidone, the preparation has the advantages of effectively lowering certain non-specific adsorption of the antibody, lowering background value and improving detection sensitivity.

Description

technical field [0001] The invention relates to a serum sample processing preparation, in particular to a serum sample processing preparation for protein suspension chip detection. Background technique [0002] With the widespread implementation of the global biosafety strategy, the demand for rapid diagnosis of pathogenic organisms has increased significantly, such as classic detection methods such as various PCR for detection of nucleic acids, in situ hybridization, DNA chips, NASBA and other technologies, detection of proteins and other antigens, antibodies Substance ELISA, immunochromatography technology, protein chip technology, etc., all have their own characteristics. However, the general method mainly detects a single pathogen or infectious disease, and it is difficult to simultaneously screen for multiple causes in the face of an infected person. [0003] The basic principle of suspension chip detection is to use microspheres made of polystyrene (polystyrene) to co...

AI Technical Summary

Problems solved by technology

However, due to the complexity and variety of antibody components in serum, severe non-specific adsorption often occurs when using antigen-coated microspheres to detect antibodies, which seriously affects the detection sensitivity. Therefore, it is necessary to prepare an effective Sample Treatment Preparations to Reduce Non-Specific Adsorption in Serum

[0005] The principle of the protein suspension chip for detecting antibodies is based on the characteristic reaction of antigens and antibodies in immunology. The method used for detecting antibodies in immunology includes enzyme-linked immunosorbent assay (ELISA) indirect method for testing antibodies, serum used The sample treatment solution is PB or PBS solution. When PB or PBS solution is used for the serum sample treatment solution detected by the suspension chip, the non-specific adsorption is serious, and false positives and weak positives are prone to occur, mainly because human serum contains about 40% The above heterophilic antibodies are prone to non-specific adsorption with microspheres, resulting in false positives and weak positives