Unlock instant, AI-driven research and patent intelligence for your innovation.

Listonella anguillarum subunit vaccine antigenic protein and application thereof

A technology of Listonia anguillaris and subunit vaccines, applied in the field of immunology, to achieve the effects of increasing immune synergy, broadening the vaccine protection spectrum, and simple preparation methods

Active Publication Date: 2011-08-03
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the immune protection effect of HCP protein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Listonella anguillarum subunit vaccine antigenic protein and application thereof
  • Listonella anguillarum subunit vaccine antigenic protein and application thereof
  • Listonella anguillarum subunit vaccine antigenic protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] According to the nucleotide sequence of the Listonia anguillarum T6SS matrix protein gene lahcp, and the restriction map design amplification primer of vector pETDuet (purchased from Novagen):

[0022] lahcp-for-EcoRI:5'-GGC GAATTC ATGCCAACTGCATG-3' (underlined base sequence is the recognition site of restriction endonuclease EcoRI);

[0023] lahcp-rev-SalI:5'-ATCG GTC GAC TTACGCTTCAACAG3' (the base sequence underlined is the recognition site of restriction endonuclease SalI).

[0024] The PCR amplification reaction was carried out using the Genomic DNA of Listonia anguillarum as a template (the PCR instrument was purchased from Takara Company, model TP600), and the reaction system and reaction parameters were as follows: (Reagents should be expressed in Chinese as much as possible)

[0025] PCR reaction system (25μL)

[0026] 10×PCR buffer 2.5μL

[0027] dNTP mixture 0.5μL (each 0.2mM)

[0028] MgCl 2 2.0μL (1.5mM)

[0029] Primer lahcp-for-Ec...

Embodiment 2

[0106] Mix the antigenic protein of the Listeria anguillaris subunit vaccine with complete Freund's adjuvant at a ratio of 1:1 by volume, and then intraperitoneally inject it into the turbot with an inoculation dose of 2 μg protein / g fish body weight .

Embodiment 3

[0108] Mix the antigenic protein of the Listeria anguli subunit vaccine with complete Freund's adjuvant in a ratio of 1:1 by volume, and then intraperitoneally inject it into the blue eel dragon with an inoculation dose of 20 μg protein / g fish body weight .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of immunology, in particular to Listonella anguillarum subunit vaccine antigenic protein and the application thereof. Listonella anguillarum lahcp gene nucleic acid base sequence is disclosed by a sequence list SEQ IDNO: 1, and coded protein is disclosed by a Listonella anguillarum subunit vaccine antigenic protein amino acid sequence list SEQ IDNO: 2. The Listonella anguillarum subunit vaccine antigenic protein can stimulate the immunity of a fish body to the Listonella anguillarum disease. The expression protein of the invention can stimulate the fish bodyto generate a specific antibody to resist the infection of the Listonella anguillarum, can be used for preventing diseases caused by the Listonella anguillarum in fish aquiculture, has a simple preparation method and can be used for large-scale industrialized production.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a subunit vaccine antigen protein of Listonia anguillarum and its application. Background technique [0002] Listonella anguillarum is a Gram-negative rod-shaped bacterium that is pathogenic to freshwater and marine farmed fish and wild fish, causing hemorrhagic septicemia in fish, causing worldwide significant economic loss. In my country, Listonia eel is not only the pathogen of red leg disease of cultured prawns, but also the pathogen of skin and muscle ulcers and hemorrhagic diseases of fish such as seabass, flounder, turbot, grouper and European eel. Fish are dangerous. [0003] Type VI Secret System (Type VI Secret System, T6SS) is a newly identified new bacterial secretion system, which is involved in the pathogenicity of bacteria and has been found in many plant or animal pathogens. Current research shows that the T6SS gene cluster structure of Gram-negative bacteria is relati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/195A61K39/02A61P31/04
Inventor 肖鹏莫照兰王波李杰
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI