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Gene chip and application thereof

A chip and small RNA technology, applied in the field of gene chips, can solve problems such as tumor occurrence

Inactive Publication Date: 2009-10-14
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies have shown that many miRNAs are involved in biological processes such as development, differentiation, growth, and immune response, and their expression and dysfunction may lead to various pathological phenomena such as tumorigenesis, leukemia, and viral infection.

Method used

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  • Gene chip and application thereof
  • Gene chip and application thereof
  • Gene chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Verification example

[0075] Materials and Methods

[0076] Case Organization:

[0077] 78 pairs of cancer and paracancerous tissues were obtained from surgical resection specimens of patients with primary liver cancer. 10 cases of normal liver from accidental death. All tissue samples obtained were approved by the ethics committee of the World Health Organization cooperative organization authorized by the Shanghai Municipal Government. Surgical resection specimens were pathologically analyzed, and pathological grades were in accordance with Edmonson's criteria. Clinical data including: age, gender, tumor size, pathological grade, lymph node metastasis, liver cirrhosis status, HBV and HCV infection were obtained from the patient's case records. All patients did not receive chemotherapy before operation, and the postoperative follow-up was up to 48 hours.

[0078] MicroRNA chip:

[0079] The probes were designed for mature miRNAs sequences, and the miRNAs sequence...

Embodiment 2

[0105] Liver Cancer Susceptibility Detection Kit

[0106] As described in Example 1, the small ribonucleic acid shown in the sequence SEQ ID NO: 1-84 is closely related to liver cancer. Therefore, detection can be performed based on this extracted patient's miRNA.

[0107] Prepare a kit (100 person-times), which contains miRNA chips, on which are immobilized specific probes for small ribonucleic acids, and the small ribonucleic acids are respectively shown in the sequences SEQ ID NO: 1-84.

[0108] A test group composed of 100 people was randomly selected, including subjects who were unknown whether they had liver cancer, patients who were known to have liver cancer, and normal people who were tested to be free of liver cancer.

[0109] Obtain the liver tissue of the subject to be detected in the test group, use the method as in Example 1 to extract RNA and prepare the test sample, and hybridize with the chip in the liver cancer detection kit to obtain the expression profile....

Embodiment 3

[0115] Auxiliary detection of susceptibility to liver cancer

[0116] The test in Example 2 was repeated, with the difference that 70 people (whose symptoms of liver cancer were not known before the test) were randomly selected for testing.

[0117] Prepare a kit (100 person-times), which contains miRNA chips, on which are immobilized specific probes for small ribonucleic acids, and the small ribonucleic acids are respectively shown in the sequences SEQ ID NO: 1-84.

[0118] Obtain the liver tissue of the subject to be detected in the test group, use the method as in Example 1 to extract RNA and prepare the test sample, and hybridize with the chip in the liver cancer detection kit to obtain the expression profile.

[0119] The results also confirmed that the susceptibility ratio of the subjects whose expression profiles were more similar to those in Example 1 (concordance rate ≥ 90%) was significantly higher than that of the population with a lower degree of similarity (concor...

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Abstract

The invention discloses a gene chip and an application thereof. The invention discloses a micro ribonucleic acid specifically expressed in liver cancer tissues and an application of the same. The gene chip is a chip which contains a probe specific to the micro ribonucleic acid and can be used for preparing reagent or a kit for differentiating liver cancer tissues, tissues adjacent to carcinoma and / or normal tissues, for preparing reagent or kit for testing liver cancer and for the auxiliary diagnosis of liver cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gene chip containing a probe specific for small ribonucleic acid and related applications thereof. Background technique [0002] Liver cancer is one of the most common tumors in Southeast Asia and South Africa, and its mortality rate is also very high. Many human diseases are caused by disordered or out-of-control gene expression. Small ribonucleic acid (small RNA, microRNA, miRNA) is a kind of single-stranded small molecule RNA with a length of about 21-25nt that does not code for protein. It exists widely in eukaryotes and binds to specific target mRNA through nucleic acid sequence complementarity. It is a molecule that plays a negative regulatory role by regulating the translation of target mRNA or degrading target mRNA. Current studies have shown that many miRNAs are involved in biological processes such as development, differentiation, growth, and immune response, and their ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/113
Inventor 何祥火顾健人杨胜利万大方李文晰
Owner SHANGHAI INST OF ONCOLOGY
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