Hyperbranched polyamino acid, preparation method and application thereof

Abstract

The invention relates to hyperbranched polyamino acid. The chemical composition is as follows: in the formula of (Lys-)a-(-Arg-)b-(-His-)c-(-X)d, Lys, Arg and His are respectively lysine, arginine and histidine, X is one of or a plurality of any amino acids except for the amino acids above, wherein a is not less than 0.2 and not more than 0.95, b is not less than 0 and not more than 0.8, c is not less than 0 and not more than 0.8, d is not less than 0 and not more than 0.3, and the sum of a, b, c and d is 1. The preparation method is as follows: uniformly mixing lysine or lysine salt with other amino acids or the amino acid salts contained in the hyperbranched polyamino acid to react for 2-64h at the temperature of 130-180 DEG C and then obtain yellow solid, and finally purifying the coarse product with a precipitation method to obtain the hyperbranched polyamino acid. The hyperbranched polyamino acid obtained in the invention can be used as nonviral gene carrier for the transduction of huamn or animal cell gene, and the hyperbranched polyamino acid nonviral gene carrier provided by the invention is reasonable in design, simple in preparation method, high in transfection efficiency, low cytotoxicity and favorable transduction efficiency in the presence of blood serum.

Description

technical field The invention relates to a non-viral gene carrier, in particular to a non-viral gene carrier whose chemical composition is hyperbranched polyamino acid, a preparation method and application thereof. Background technique Gene therapy is to deliver the target gene to specific tissue cells (target cells) in the patient's body through a suitable carrier for proper expression, so as to correct or improve the defects caused by the disease-causing gene and achieve the purpose of treating the disease. Gene therapy has made great progress in the development of half a century, but also encountered many difficulties. At present, before gene therapy enters the practical stage, there are still some fundamental problems to be solved. Among them, the construction of safe and efficient gene vectors is one of the most important issues. In general, gene delivery systems fall into two categories, viral vector systems and non-viral vector systems. Non-viral vectors mainly rel...

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Problems solved by technology

However, the complex preparation steps and high preparation cost limit the application of dendrimers.

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