Virulent phage sensitive to riemerella anatipestifer and application thereof

A technology of Reesei and bacteriophage, applied in virus/phage, medical raw materials derived from virus/phage, antibacterial drugs, etc., can solve the problems of adverse ecological environment, environmental pollution of drugs, veterinary drug residues, etc.

Inactive Publication Date: 2009-11-11
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Farmers often use drugs for treatment, but currently there are not many drugs that are sensitive to R. anatipestifer and are prone to drug resistance. Therefore, it is not uncommon for multiple drugs to be overdose or over the course of treatment. The consequences, on the one hand, lead to duck carcass products The residues of veterinary drugs will affect human health after being ingested into the human body; on the other hand, the excrement of duck farms is discharged to the surrounding environment, and the drugs become environmental pollutants, which have adverse effects on the ecological environment

Method used

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  • Virulent phage sensitive to riemerella anatipestifer and application thereof
  • Virulent phage sensitive to riemerella anatipestifer and application thereof
  • Virulent phage sensitive to riemerella anatipestifer and application thereof

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[0011] The R. anatipestifer RAf71 that can be lysed by the phage was cultured first, and the medium was trypticase soybean agar supplemented with 10 mL / L newborn calf serum, and the culture condition was 24 hours in a candle jar at 37°C. Wash the bacteria sludge with a sterile 10mM magnesium sulfate solution, adjust the OD525nm value to 0.4, take 100 microliters and mix it with an appropriate amount of RAP37, bathe in 37°C water for 15min, add 45°C 7g / L agar, and immediately spread evenly on the 10mL / L On the trypticase soybean agar of newborn bovine serum, after the agar solidified, it was cultured in a candle jar at 37°C for 24 hours, and transparent plaques of phages were visible, with a diameter of about 1-2 mm and no halos around. The phage consists of a polyhedral head and a slender tail. The plane profile of the head is hexagonal, with a diameter of about 60nm, and the tail is about 200nm long and 8nm wide. Its genome is double-stranded DNA. The virulent phage sensitive...

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Abstract

The invention discloses a virulent phage sensitive to riemerella anatipestifer and application thereof. The virulent phage sensitive to riemerella anatipestifer is named RAP37 and is preserved in China General Microbiological Culture Collection Center with preservation date of March-2-2009 and preservation number of CGMCC No.2908, the diameter thereof is about 1-2mm, the periphery is not provided with halo, the phage is composed of one polyhedral head and lathy tail, the face profile of the head is hexagon and the diameter is about 60nm, the tail is about 200nm long and 8nm wide and the genome thereof is double-stranded DNA. The virulent phage sensitive to riemerella anatipestifer is named RAP37, can decompose the riemerella anatipestifer, and can treat and prevent riemerella anatipestifer disease caused by leading pathogen of the riemerella anatipestifer which endangers duck breeding industry by adopting biotherapy.

Description

【Technical field】 [0001] The present invention relates to a kind of microorganism and its application. 【Background technique】 [0002] Riemerella anatipestifer (RA) disease is a major bacterial infectious disease that endangers the duck industry. The prevention and control measures of R. anatipestifer include vaccination and drug treatment. The existing vaccines are inactivated vaccines, which have the advantages of safety and low development costs, but often fail to achieve good results, because the serotypes of the prevailing strains often change, resulting in immune failures from time to time. Farmers often use drugs for treatment, but currently there are not many drugs that are sensitive to R. anatipestifer and are prone to drug resistance. Therefore, it is not uncommon for multiple drugs to be overdose or over the course of treatment. The consequences, on the one hand, lead to duck carcass products The residues of veterinary drugs will affect human health after being ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
Inventor 程龙飞黄瑜郑腾傅光华施少华陈红梅万春和彭春香
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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