Hexanol degrading bacterium and preparation method and application thereof

A technology of degrading and geomycosis, applied in the field of microorganisms, can solve problems such as difficult production, achieve great application potential, and improve the effect of separation and screening.

Inactive Publication Date: 2009-12-16
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these are all metabolic pathways in plants and are difficult to use in production

Method used

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  • Hexanol degrading bacterium and preparation method and application thereof
  • Hexanol degrading bacterium and preparation method and application thereof
  • Hexanol degrading bacterium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The isolation of embodiment 1TF bacterial strain, identification method and biological characteristic thereof

[0028] 1. Materials and methods

[0029] 1.1 Main instruments and reagents

[0030] GC9800 Gas Chromatograph Analyzer (Shanghai Kechuang Chromatographic Instrument Co., Ltd.), PYX-DHS-40x50 Water-proof Electric Heating Constant Temperature Incubator (Shanghai Yuejin Farm Medical Equipment Factory), SPX-300B-II Biochemical Incubator (Shanghai Yuejin Medical Instrument Factory) factory), HWY single-layer large-capacity full-temperature constant temperature oscillator (Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.), SW-CJ-2FD clean bench (Suzhou Antai Air Technology Co., Ltd.), Motic BA 400 digital imaging optical microscope (attached Motic 3.1 Preview Software). N-hexanol (chromatographically pure) (imported by Sinopharm Chemical Reagent Co., Ltd.).

[0031] 1.2 Medium

[0032] Strain acclimatization and enrichment culture medium: peptone 1...

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Abstract

The invention discloses Galactomyces geotrichum T3d329TF which is obtained by directional induction, cultivation, domestication, selection and separation in natural environment. The Galactomyces geotrichum T3d329TF is preserved in the China Center for Type Culture Collection (CCTCC) on October 21, 2008, and the preserving number is CCTCC NO: M208167. The Galactomyces geotrichum T3d329TF has the action of degrading hexanol and can generate various aroma components simultaneously.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a n-hexanol-degrading bacterium, a preparation method thereof, and an application thereof in degrading n-hexanol and producing aroma. Background technique [0002] n-hexanol is a type of fusel oil [1] When the content of fusel oil is high, it will cause adverse symptoms such as wine topping [2] . At present, the chemical conversion pathways of n-hexanol are: n-hexanol is converted into n-pentanoic acid [3] ; n-hexanol is converted to n-hexanoic acid [4] or converted to n-hexanal [5][6] ; N-hexanol natural products exist in apples, bitter oranges, strawberries, tea leaves, violets, lavender, etc., and its biological metabolic pathways are: n-hexanol is converted into n-hexanal by the action of lipoxygenase (LOX), [7][8] , n-hexanol can generate hexyl acetate through the action of alcohol acyltransferase (AAT) and acetic acid [9] . However, these are metab...

Claims

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Application Information

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IPC IPC(8): C12N1/14C11B9/00C12R1/645
Inventor 师俊玲令桢民杨保伟
Owner NORTHWEST A & F UNIV
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