Hexanol degrading bacterium and preparation method and application thereof

A kind of n-hexanol, degradation technology, applied in the field of microorganisms

Inactive Publication Date: 2011-03-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these are all metabolic pathways in plants and are difficult to use in production

Method used

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  • Hexanol degrading bacterium and preparation method and application thereof
  • Hexanol degrading bacterium and preparation method and application thereof
  • Hexanol degrading bacterium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification methods of TF strains and their biological characteristics

[0030] 1. Materials and methods

[0031] 1.1 Main instruments and reagents

[0032] GC9800 Gas Chromatography Analyzer (Shanghai Kechuang Chromatography Instrument Co., Ltd.), PYX-DHS-40x50 Waterproof Electric Heating Constant Temperature Incubator (Shanghai Yuejin Farm Medical Equipment Factory), SPX-300B-II Biochemical Incubator (Shanghai Yuejin Medical Equipment Co., Ltd.) Factory), HWY single-layer large-capacity full-temperature constant temperature oscillator (Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.), SW-CJ-2FD clean workbench (Suzhou Antai Air Technology Co., Ltd.), Motic BA 400 digital imaging optical microscope (attached Motic3.1 Preview Software). n-Hexanol (chromatographically pure) (imported and subpackaged by Sinopharm Chemical Reagent Co., Ltd.).

[0033] 1.2 Culture medium

[0034] Bacteria acclimation and enrichment culture medi...

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PUM

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Abstract

The invention discloses Galactomyces geotrichum T3d329TF which is obtained by directional induction, cultivation, domestication, selection and separation in natural environment. The Galactomyces geotrichum T3d329TF is preserved in the China Center for Type Culture Collection (CCTCC) on October 21, 2008, and the preserving number is CCTCC NO: M208167. The Galactomyces geotrichum T3d329TF has the action of degrading hexanol and can generate various aroma components simultaneously.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a n-hexanol-degrading bacterium, a preparation method thereof, and an application thereof in degrading n-hexanol and producing aroma. Background technique [0002] n-hexanol is a type of fusel oil [1] When the content of fusel oil is high, it will cause adverse symptoms such as wine topping [2] . At present, the chemical conversion pathways of n-hexanol are: n-hexanol is converted into n-pentanoic acid [3] ; n-hexanol is converted to n-hexanoic acid [4] or converted to n-hexanal [5][6] ; N-hexanol natural products exist in apples, bitter oranges, strawberries, tea leaves, violets, lavender, etc., and its biological metabolic pathways are: n-hexanol is converted into n-hexanal by the action of lipoxygenase (LOX), [7][8] , n-hexanol can generate hexyl acetate through the action of alcohol acyltransferase (AAT) and acetic acid [9] . However, these are metab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/645C12N1/14C11B9/00
Inventor 师俊玲令桢民杨保伟
Owner NORTHWEST A & F UNIV
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