Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof
A technology of red gland honeysuckle and honeysuckle, applied in the field of drug analysis, to achieve good reproducibility, stable quality, and strong intuitive effect
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[0021] 1 Instruments and reagents
[0022] 1.1 Instrument: Dionex high performance liquid chromatography (ASI-100 autosampler, ATH-585 column thermostat, P680 quaternary gradient pump, PDA-100 detector);
[0023] Column: Dikma PLATISIL ODS C 18 , 250mm×4.6mm, 5μm;
[0024] Prefabricated silica gel H sheet and G sheet (glass substrate 20×20cm, Luqiao Sijia Biochemical Plastic Factory, Taizhou City, Zhejiang Province);
[0025] CAMAG AUTOMATIC TLC SAMPLER thin-layer automatic spotting instrument, CAMAG TLCREPROSTAR thin-layer automatic imaging system, CAMAG double-slot expansion cylinder (Swiss CAMAG company);
[0026] 1.2 Test drug: 10 batches of honeysuckle, Kouyanqing granule, and honeysuckle control drug were all provided by Guangzhou Baiyunshan Hutchison Whampoa Traditional Chinese Medicine Co., Ltd. The acetonitrile reagent used in the liquid chromatography in the experiment is chromatographically pure, the rest of the reagents used are analytically pure, and the water ...
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