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Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof

A technology of red gland honeysuckle and honeysuckle, applied in the field of drug analysis, to achieve good reproducibility, stable quality, and strong intuitive effect

Active Publication Date: 2010-01-20
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the difficulties of the prior art, and the purpose is to provide an efficient and reliable method for identifying honeysuckle and honeysuckle

Method used

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  • Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof
  • Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof
  • Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof

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Experimental program
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Embodiment 1

[0021] 1 Instruments and reagents

[0022] 1.1 Instrument: Dionex high performance liquid chromatography (ASI-100 autosampler, ATH-585 column thermostat, P680 quaternary gradient pump, PDA-100 detector);

[0023] Column: Dikma PLATISIL ODS C 18 , 250mm×4.6mm, 5μm;

[0024] Prefabricated silica gel H sheet and G sheet (glass substrate 20×20cm, Luqiao Sijia Biochemical Plastic Factory, Taizhou City, Zhejiang Province);

[0025] CAMAG AUTOMATIC TLC SAMPLER thin-layer automatic spotting instrument, CAMAG TLCREPROSTAR thin-layer automatic imaging system, CAMAG double-slot expansion cylinder (Swiss CAMAG company);

[0026] 1.2 Test drug: 10 batches of honeysuckle, Kouyanqing granule, and honeysuckle control drug were all provided by Guangzhou Baiyunshan Hutchison Whampoa Traditional Chinese Medicine Co., Ltd. The acetonitrile reagent used in the liquid chromatography in the experiment is chromatographically pure, the rest of the reagents used are analytically pure, and the water ...

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Abstract

The invention discloses a method for distinguishing lonicera hypoglauca miq and honeysuckle, which distinguishes the lonicera hypoglauca miq and the honeysuckle by utilizing thin-layer chromatography. The method further distinguishes the lonicera hypoglauca miq and the honeysuckle by utilizing high performance liquid chromatography. The distinguishing method can be used for evaluating real or fake lonicera hypoglauca miq or honeysuckle and the quality of both, used for distinguishing lonicera hypoglauca miq medicine material and honeysuckle medicine material, and also can be used for quality evaluation of feeding medicine materials in preparations so as to ensure the stable, uniform and controllable quality of the preparations. The method has the advantages of simple and convenient operation, good repeatability, strong visual impression and the like.

Description

technical field [0001] The invention discloses a method for distinguishing honeysuckle and honeysuckle and its application, and belongs to the field of drug analysis. Background technique [0002] Red glandular honeysuckle was recorded as a source of honeysuckle in the previous editions of the 2005 edition of "The Pharmacopoeia of the People's Republic of China". The same, similar species, same taste, identification, function and indications, usage and dosage, the content determination items are high-performance liquid chromatography to determine the content of chlorogenic acid, the method and limit are the same, the difference is that honeysuckle adds the content determination of luteolin item, but it is reported in the literature that honeysuckle also contains luteolin. According to the literature reports, the main reason for the standard classification is that "multi-source varieties with large differences in the components of Chinese herbal medicines are gradually class...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/35G01N30/36G01N30/90A61K133/00
Inventor 苏薇薇李楚源关倩怡王德勤彭维林青王永刚黄琳梁峰肖晓丽
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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