Method for preparing female silkworm moth powder with high estradiol content

[0017] Example 1:

[0018] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after the pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 4 μg/head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours, the female silkworm chrysalis will emerge after being exposed to light. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30 within 2 hours. Freeze at ℃. The frozen female silkworm moth was vacuum-dried at -50°C for 36 hours, the wings and feet were cut off, and then superfinely pulverized at -15°C for 10 minutes, and then passed through a 300-mesh sieve to obtain the silkworm female moth powder. The estradiol content in Bombyx moth powder is 3786pg/g, which can significantly increase the uterine coefficient, vaginal coefficient and serum estradiol level of bilateral ovariectomized model rats. returned to normal levels. The same results were obtained in all 6 batches tested.

[0019] Example 2:

[0020] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 5 μg/head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours of exposure, the female silkworm pupae will emerge. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30°C within 4 hours. freezing. The frozen female silkworm moth was vacuum-dried at -50°C for 36 hours, the wings and feet were cut off, and then superfinely pulverized at -15°C for 10 minutes, and then passed through a 400-mesh sieve to obtain silkworm female moth powder. The estradiol content in Bombyx moth powder is 3875pg/g, which can significantly increase the uterine coefficient, vaginal coefficient and serum estradiol level of bilateral ovariectomized model rats. returned to normal levels. The same results were obtained in all 8 batches tested.

[0021] Example 3:

[0022] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 5 μg/head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours, the female silkworm chrysalis will emerge after being exposed to light. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30 within 2 hours. Freeze at ℃. The frozen female silkworm moth was vacuum-dried at -50°C for 36 hours, the wings and feet were cut off, and then superfinely pulverized at -15°C for 10 minutes, and then passed through a 300-mesh sieve to obtain the silkworm female moth powder. The estradiol content in silkworm female moth powder is 3851pg/g, which can significantly improve the uterine coefficient, vaginal coefficient and serum estradiol level of bilateral ovariectomized model rats. returned to normal levels. The same results were obtained in all 7 batches tested.

[0023] There are many varieties of silkworm. The above is the experimental result of the 54A variety. If it is replaced with other silkworm varieties, according to the same technical process, the result of estradiol content (3700-3900 pg/g) can also be obtained.