Method for preparing female silkworm moth powder with high estradiol content

A technology with high estradiol and estradiol, which is applied in the field of preparation of silkworm female moth powder, achieves positive social and economic benefits, meets diversified needs, and has remarkable therapeutic effects

Inactive Publication Date: 2010-02-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hormone replacement therapy is a method that uses exogenous estrogen or estrogen combined with progesterone to delay or alleviate women's menopause syndrome. Although its curative effect has

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1:

[0018] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after the pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 4 μg / head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours, the female silkworm chrysalis will emerge after being exposed to light. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30 within 2 hours. Freeze at ℃. The frozen female silkworm moth was vacuum-dried at -50°C ...

Example Embodiment

[0019] Example 2:

[0020] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 5 μg / head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours of exposure, the female silkworm pupae will emerge. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30°C within 4 hours. freezing. The frozen female silkworm moth was vacuum-dried at -50°C for 36 hours, the wings an...

Example Embodiment

[0021] Example 3:

[0022] In spring, use suitable mature mulberry leaves at 25-28°C (27-28°C for 1-2 instars, 26°C for 3-4 instars, and 25°C for 5-instars) in a clean environment. 24~25 ℃ of protection, open the cocoon after pupation, take out the silkworm pupae and carry out male and female identification, get the female silkworm pupae and inject 20-hydroxyecdysone (dose is 5 μg / head) subcutaneously in the abdomen on the second day of pupation, 24 days before eclosion. The female silkworm pupae were evenly sprayed with 75% ethanol solution for 3 hours and sealed for 3 hours. Continue to protect from black at 24-25°C. After 21 hours, the female silkworm chrysalis will emerge after being exposed to light. After 5 hours of eclosion, the female silkworm moth will be vibrated to empty the moth urine, and then moved into a refrigerator, and the temperature will be gradually lowered to -30 within 2 hours. Freeze at ℃. The frozen female silkworm moth was vacuum-dried at -50°C for ...

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PUM

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Abstract

The invention discloses a method for preparing female silkworm moth powder with high estradiol content, which comprises the following steps: raising a silkworm by mulberry leaves till the silkworm climbs a cluster and cocoons; after the silkworm is pupated, opening a cocoon; taking a silkworm pupa out to carry out sex authentication; protecting a female silkworm pupa in the normal environment; inthe second day of pupation, hypodermically injecting 20-hydroxy ecdysterone to the abdomen of the female silkworm pupa, wherein each female silkworm pupa is injected with 4-5 micrograms of 20-hydroxyecdysterone; in 24 hours before eclosion, uniformly spraying a silkworm pupa body by 75 percent of alcohol liquid and sealing for 3 hours; in 5 hours after eclosion, freezing, drying, ultramicro-pulverizing and preparing the female silkworm moth powder with high estradiol content. The prepared female silkworm moth powder has high estradiol content, obvious therapeutic effect on a climacteric syndrome and positive social and economic benefits, can be used for developing a treating medicine or health food for preventing and treating the woman climacteric syndrome so as to meet the diversified requirement at home and abroad for the treating medicine or the health food of the woman climacteric syndrome and can improve the traditional sericulture.

Description

technical field [0001] The invention relates to a preparation method of silkworm female moth powder with high estradiol content. Background technique [0002] Menopausal syndrome is a group of symptoms caused by the decrease of estrogen secretion in women, which causes endocrine disorders and autonomic disorders. Symptoms such as instability, insomnia, forgetfulness, irritability and suspiciousness, fatigue and cramps, osteoporosis, dull skin, increased cholesterol, and increased psychological stress not only seriously endanger physical and mental health, but also seriously affect work, husband and wife relationships, and family happiness. With the acceleration of the pace of human life and the deepening of aging, in recent years, the age of menopause has become younger and the number of menopausal syndromes has increased rapidly, which has become a global medical topic and social public health problem. [0003] At present, clinical treatment of menopausal syndrome mainly i...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P15/12A61P5/30A01K67/04
Inventor 时连根李有贵顾美儿贺伟强金卫明费建明李玉峰
Owner ZHEJIANG UNIV
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