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Method for embedding wax tissue chip

A tissue chip and paraffin technology, applied in the field of paraffin tissue chip embedding, can solve the problems of limited aperture, limited shape, and inability to sample at any time, and achieve the effect of promoting promotion and sufficient sources

Inactive Publication Date: 2010-04-14
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Second, the preparation of tissue chips requires specialized technicians;
[0009] Third, the preparation of the tissue chip can only be carried out after all the wax block specimens are collected. In daily work, samples cannot be taken at any time and embedded at any time.
[0010] Fourth, the recipient wax block must be punched with a tissue chip machine, and a tissue chip machine has a limited aperture and a limited shape (only circular)

Method used

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  • Method for embedding wax tissue chip
  • Method for embedding wax tissue chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. Preparation of tissue chip memory board:

[0027] Such as figure 1 As shown, it is a schematic diagram of a tissue chip storage board. On a transparent hard plastic board 1 with a thickness of 6 mm to 10 mm, within a range of 50 mm in length and 25 mm in width, engrave length b: 1 mm to 5 mm, width a: 1 mm to 5 mm, and depth d : 2-3mm square or rectangular grooves constitute the groove array 2, and a range of 5mm-10mm is reserved around the transparent hard plastic plate 1 for marking row numbers (such as A, B, C, D...) and The number of rows (such as 1, 2, 3, 4...) is convenient for organizing chip positioning. Or grooves with different lengths and widths are arranged in a separate row for combination. Used to store tissue chips during embedding.

[0028] Preparation of tissue chip embedding plate:

[0029] Such as figure 2 As shown, it is a schematic diagram of a tissue chip embedding plate. The heat-resistant plate 4 can be made of plastic (or plastic, glass...

Embodiment 2

[0036] Similar to Example 1, the difference is that in the step of embedding the tissue chip with the tissue chip embedding plate, put the paraffin into the paraffin pot, heat it to 70°C with an electric furnace to melt the paraffin; add the melted paraffin into the wax model along one end of the wax model until it is filled with the wax model, let stand at 25°C for 10 minutes to allow the paraffin to polymerize, and then remove the wax model; separate the tissue chip embedding plate from the paraffin to obtain a wax block containing the tissue chip, and place the tissue chip side of the wax block toward Place it on the ironing table until the bottom surface is flat, and slice along the direction parallel to the flat surface of the tissue chip.

Embodiment 3

[0038] Similar to Example 1, the difference is that in the step of embedding the tissue chip with the tissue chip embedding plate, put the paraffin into the paraffin pot, and heat it to 65°C with an electric furnace to melt the paraffin; add the melted paraffin into the wax model along one end of the wax model until it is filled with the wax model, let stand at 20°C for 20 minutes to allow the paraffin to polymerize, and then remove the wax model; separate the tissue chip embedding plate from the paraffin to obtain a wax block containing the tissue chip, and place the tissue chip side of the wax block toward Place it on the ironing table until the bottom surface is flat, and slice along the direction parallel to the flat surface of the tissue chip.

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Abstract

The invention provides a method for embedding wax tissue chip, which is characterized in that the method comprises the detailed steps of cutting small tissue blocks on the wax tissue, storing the small tissue blocks in a tissue chip storage plate and numbering the small tissue blocks; arranging the tissue chip storage plate on a burning platform till the wax composition is melted in the small tissue blocks; taking the small tissue blocks out of the tissue chip storage plate and arranging the small tissue blocks in a tissue chip embedding plate; arranging the tissue chip embedding plate in a rectangular wax mold assembled by two vertically bent copper bars; taking the wax out and arranging the wax into a wax kettle; heating and melting the wax by an electric furnace; adding the melted wax into the wax mold along one end of the wax mold till the wax mold is fully filled; removing the wax mold, separating the tissue chip embedding plate from the wax; and slicing along the direction in parallel to the even surface of the tissue chip. The method has the advantages of being low in cost, conveniently, quickly and freely collecting the chip sample, freely storing the samples in the storage plate, and having repeatable usage.

Description

technical field [0001] The invention relates to a paraffin tissue chip embedding method, belonging to the technical field of tissue chips. Background technique [0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can be combined with DNA, RNA, protein, antibody and other technologies, combined with traditional pathology technology, histochemistry and immunohistochemistry technology, and can be carried out...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 侯英勇卢韶华侯君宿杰·阿克苏曾海英刘弢高峰谭云山
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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