Full-automatic cell physiological parameter analyzer based on multi-parameter detection integrated chip

Abstract

The invention discloses a full-automatic cell physiological parameter analyzer based on a multi-parameter detection integrated chip. The full-automatic cell physiological parameter analyzer comprises a sealed measurement cavity, wherein a chip interface device and a multi-parameter integrated chip component are internally arranged in the measurement cavity; the chip interface device comprises a base, a chip interface circuit board and a sealing cap which are sequentially and fixedly connected into a whole from bottom to top; a through-hole fitted with the multi-parameter integrated chip component is formed in the center of the sealing cap; a pressure gasket is connected above the through-hole of the sealing cap; a spring ejector pin is welded on the chip interface circuit board; the multi-parameter integrated chip component is arranged in the through-hole of the sealing cap and forms close contact among the pressure gasket, the multi-parameter integrated chip component and the spring ejector pin in sequence; a computer and a light-addressable potentiometric sensor (LAPS) detection circuit, an impedance detection circuit and a microelectrode array detection circuit of a measuring and controlling circuit are connected with a central controlling circuit respectively; and the LAPS detection circuit, the impedance detection circuit and the microelectrode array detection circuit are connected with the chip interface circuit board respectively.

Description

technical field

[0001] The invention relates to a cell physiological parameter analyzer. Background technique

[0002] The extracellular signal detection method has attracted more and more attention because it can realize non-destructive and long-term measurement, and the development of related technologies is also changing rapidly, such as microelectrode array (MEA), light addressable potential sensor (LAPS), cell resistance Anti-measurements, etc. are also being developed towards commercialization. However, when performing cell physiology experiments, the monitoring of a single parameter often can only reflect the physiological activities of the cells from a certain aspect, and the complexity of the test environment (various noise sources, corrosion of the electrode by the culture solution after a long-term test, etc.) ) will interfere with the results of the test, so the reveal of the mechanism of cell physiological activities requires a comprehensive analysis of multipl...

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