Full-automatic cell physiological parameter analyzer based on multi-parameter detection integrated chip

An integrated chip and physiological parameter technology, applied in the field of cell physiological parameter analyzers, can solve the problems of synchronous detection and comprehensive analysis of multiple physiological parameters of cells without an analyzer, and achieve the effect of less sample consumption, high efficiency and short analysis time

CN101705184AInactive Publication Date: 2010-05-12ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2010-05-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a full-automatic cell physiological parameter analyzer based on a multi-parameter detection integrated chip. The full-automatic cell physiological parameter analyzer comprises a sealed measurement cavity, wherein a chip interface device and a multi-parameter integrated chip component are internally arranged in the measurement cavity; the chip interface device comprises a base, a chip interface circuit board and a sealing cap which are sequentially and fixedly connected into a whole from bottom to top; a through-hole fitted with the multi-parameter integrated chip component is formed in the center of the sealing cap; a pressure gasket is connected above the through-hole of the sealing cap; a spring ejector pin is welded on the chip interface circuit board; the multi-parameter integrated chip component is arranged in the through-hole of the sealing cap and forms close contact among the pressure gasket, the multi-parameter integrated chip component and the spring ejector pin in sequence; a computer and a light-addressable potentiometric sensor (LAPS) detection circuit, an impedance detection circuit and a microelectrode array detection circuit of a measuring and controlling circuit are connected with a central controlling circuit respectively; and the LAPS detection circuit, the impedance detection circuit and the microelectrode array detection circuit are connected with the chip interface circuit board respectively.
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Description

technical field

[0001] The invention relates to a cell physiological parameter analyzer. Background technique

[0002] The extracellular signal detection method has attracted more and more attention because it can realize non-destructive and long-term measurement, and the development of related technologies is also changing rapidly, such as microelectrode array (MEA), light addressable potential sensor (LAPS), cell resistance Anti-measurements, etc. are also being developed towards commercialization. However, when performing cell physiology experiments, the monitoring of a single parameter often can only reflect the physiological activities of the cells from a certain aspect, and the complexity of the test environment (various noise sources, corrosion of the electrode by the culture solution after a long-term test, etc.) ) will interfere with the results of the test, so the reveal of the mechanism of cell physiological activities requires a comprehensive analysis of multipl...

Examples

Embodiment Construction

[0040] figure 1 The overall structure diagram of the whole analyzer is given. It includes a closed measuring chamber 13, which is built with a chip interface device, a multi-parameter integrated chip device 7, a temperature sensor 17, a humidity sensor 18, CO 2 Concentration sensor 19, heater 21 and humidifier 20.

[0041] The analyzer includes an automatic sampling system for culture fluid and medicine. The liquid in the culture fluid container 3 and drug container 4 flows into the surface of the multi-parameter integrated chip device 7 through the sampling pump 1 to stimulate the cells on the chip. The on-off and rotational speed of the sampling pump 1 are controlled by the full-flow analysis control circuit in the measurement and control circuit 24 to complete the automatic injection and cleaning of the liquid in the culture solution container 3 and the drug container 4, and the waste liquid passes through the sampling pump 16 It flows into the waste liquid container 16, ...