Sugarcane tissue-culture-seedling bare-seedling field-transplanting method

A technology of tissue culture seedlings and naked seedlings, which is applied in the field of reproduction and production of sugarcane seedlings, can solve the problems of large area occupied by seedling cultivation sites, long cultivation time, and high production costs, and achieves the advantages of easy seedling raising, convenient transportation, and transportation cost saving Effect

Inactive Publication Date: 2010-05-19
广西壮族自治区甘蔗研究所
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AI Technical Summary

Problems solved by technology

Reducing the artificial planting of tissue culture seedling nutrition cups can effectively solve the problems of long cultivation time in the nursery before planting sugarcane tissue culture

Method used

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  • Sugarcane tissue-culture-seedling bare-seedling field-transplanting method

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Embodiment Construction

[0016] The present invention will be further described in detail through the following specific examples, but the field transplanting time and content of the naked seedlings of the present invention are not limited thereto.

[0017] 1. Cultivate strong rooted seedlings

[0018] Using Xintaitang No. 22 as a donor, the shoot tip was detoxified from July to August, and the detoxified tissue culture seedlings were bred. The proliferation medium was MS+6-BA1.0-1.5mg / L+NAA0.1mg / L, Proliferate under the conditions of natural scattered light and temperature of 28°C. When the tissue culture seedlings proliferate to the 5th to 6th generation, they are transferred to hormone-free MS medium and cultured for about 30 days (1 to 2nd generation time), to reduce the proliferation speed, so that the nutrition of the medium can meet the existing conditions to the greatest extent. The tissue culture seedlings grew robustly. The tissue-cultured seedlings cultivated by strong seedlings took root...

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Abstract

The invention provides a sugarcane tissue-culture-seedling bare-seedling field-transplanting method which comprises the following steps: culturing sugarcane tissue culture seedlings in a sound seedling culture medium for 1-2 generations; carrying out the rooting culture; group-planting in a seedling breeding garden to breed the seedlings for 30-40 days; after each sugarcane seedling has the independent root system, hardening the group-planted seedlings, dividing the seedlings, and directly transplanting each bare individual seedling to a prepared seedling breeding field; and after planting, carrying out the management for enhancing the survival percent, and the like. Compared with the conventional tissue culture seedling breeding transplanting method, the method of the invention reduces the step of tissue-culture-seedling nutritive cup temporary planting, can effectively solve the problems of long nursery breeding time before sugarcane tissue culture seedling is planted to the field, large occupation area in the nursery, high production cost and the like, and has the advantages of easy seedling lifting, convenient transportation and transportation cost saving. The invention prolongs the field growth time and enhances the per unit yield by about 25%.

Description

technical field [0001] The invention relates to the reproduction and production of sugarcane seedlings, in particular to a field transplanting method for naked sugarcane tissue cultured seedlings. Background technique [0002] In order to speed up the popularization and propagation of new sugarcane seedlings and virus-free healthy seedlings, advanced plant tissue culture technology is used for rapid propagation of sugarcane seedlings. At present, sugarcane tissue culture seedlings generally go through the steps of sand bed cluster planting in the nursery, artificial planting of individual nutrient cups, and field transplanting during the period from rooting bottle seedlings to transplanting in the field, which is relatively unfavorable to the breeding and promotion of seedlings. Influence, the main manifestations are: (1) Nutrient cup fake seedlings take up a long time in the nursery, and the efficiency is low. From April to October when the conditions of light, temperature ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 李松余坤兴戴友铭谭芳刘丽敏刘红坚淡明陈引芝游建华杨荣仲
Owner 广西壮族自治区甘蔗研究所
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