Homocysteine diagnosing/measuring reagent (kit) and homocysteine concentration measuring method
A technology of homocysteine and cystine transhydrogenase, which is applied in biological testing, biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of long time-consuming tests, complex operations, and large variations in test data, etc. question
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Embodiment 1
[0044] The homocysteine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0045] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0046] Stabilizer 500mmol / L
[0047] Reduced coenzyme 0.25mmol / L
[0048] Glutathione Homocystine Transhydrogenase 6000U / L
[0049] Glutathione oxidase 8000U / L
[0050] NADH peroxidase 10000U / L
[0051] Glutathione disulfide 5mmol / L
[0052] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0053] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested homocysteine sample to the reagent is 1 / 25, the reaction direction is negative reaction (falling reaction), the delay time is about 0 minutes, and the detecti...
Embodiment 2
[0056] The homocysteine diagnosis / measurement reagent of this embodiment is a dual reagent, including:
[0057] Reagent 1
[0058] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0059] Stabilizer 50mmol / L
[0060] Reduced coenzyme 0.25mmol / L
[0061] Glutathione disulfide 5mmol / L
[0062] Reagent 2
[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0064] Stabilizer 500mmol / L
[0065] Glutathione Homocystine Transhydrogenase 6000U / L
[0066] Glutathione oxidase 8000U / L
[0067] NADH peroxidase 10000U / L
[0068] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0069] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested homocysteine sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the r...
Embodiment 3
[0072] The homocysteine diagnosis / measurement reagents of this embodiment are three reagents, including:
[0073] Reagent 1
[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0075] Stabilizer 50mmol / L
[0076] Reduced coenzyme 0.25mmol / L
[0077] Glutathione disulfide 5mmol / L
[0078] Reagent 2
[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0080] Stabilizer 500mmol / L
[0081] Glutathione oxidase 8000U / L
[0082] NADH peroxidase 10000U / L
[0083] Reagent 3
[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0085] Stabilizer 500mmol / L
[0086] Glutathione Homocystine Transhydrogenase 6000U / L
[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0088] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minu...
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