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Method for rejuvenating and detoxifying edible fungal strain by using protoplast regeneration technology

A protoplast and edible fungus technology, which is applied in the field of cell engineering, can solve the problems of easy degradation, aging, unstable edible fungus characters, and easy to be harmed by virus diseases, and achieves the effect of fast utilization and high utilization rate.

Inactive Publication Date: 2011-05-11
TANGSHAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, most of the edible fungi varieties cultivated in my country are through natural selection. Many edible fungi have unstable properties and are prone to degeneration and aging, making it difficult to maintain the high-quality properties of these edible fungi varieties for a long time, and are more vulnerable to viral diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Bailing mushroom white 10 protoplast rejuvenation and virus-free strain, the specific method is as follows:

[0031] 1. Parent selection: Pleurotus ostreatus 89 is an edible fungus variety cultivated all year round, but it is easy to degenerate. Degenerated hyphae grow slowly, sparsely or in bundles, uneven development, yellow mycelia, variegated spots or irregular granules appear in the strain. Mushroom production is reduced and susceptible to pests and diseases.

[0032] 2. Protoplast preparation:

[0033] Using 0.6mol / L mannitol and 2% lysozyme as the cell wall degradation solution, culture mycelia in P89 liquid at 35°C for 2.5 hours, collect the protoplasts by filtration and centrifugation, and examine the protoplasts with a microscopic plate number of bodies.

[0034] 3. Protoplast regeneration:

[0035] Protoplasts were pre-cultured in PSA medium for 12 hours, and then cultured on PSA solid medium until colonies were produced.

[0036] 4. Characteristics and ...

Embodiment 2

[0045] Pleurotus eryngii was used to replace Pleurotus eryngii 89 in Example 1, and the remaining operating steps, methods, reagent types and concentrations were the same as in Example 1. The results showed that after rejuvenation and detoxification, the strain had obvious advantages.

Embodiment 3

[0047] Bailing mushroom 10 was used to replace Pleurotus ostreatus 89 in Example 1, and the remaining operation steps, methods, reagent types and concentrations were the same as in Example 1. The results showed that the superiority of the strain was obvious after rejuvenation and detoxification.

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Abstract

The invention relates to the domain of cell engineering, in particular to a method for rejuvenating and detoxifying an edible fungal strain by using protoplast regeneration technology. The method comprises the following steps: selecting parents, preparing protoplast, regenerating the protoplast, and establishing an evaluation standard for rejuvenating and detoxifying the edible fungal strain. Thestep of preparing the protoplast comprises the step of selecting steady infiltration agent solute, steady infiltration agent concentration, degrading enzyme, enzymolysis time and enzymolysis temperature; and the step of regenerating the protoplast comprises the step of selecting a pre-culture medium and a culture method. The method has the advantages that the effects of removing viable bacteria in the edible fungal and inhibiting factor accumulation are achieved by obtaining the protoplast so as to fulfill the purposes of rejuvenating and detoxifying the edible fungal and recover good properties of the edible fungal. For example, the comprehensive properties of oyster mushroom 89 and pleurotus ferulae 10 are remarkably superior to those of a contrast variety in the aspects of yield, adaptability, commercial property, impure fungal resistance and the like, and the remarkable economic benefit is produced.

Description

Technical field: [0001] The invention relates to the field of cell engineering, in particular to a method for rejuvenating and detoxifying edible fungus strains by using protoplast regeneration technology. Background technique: [0002] At present, most of the edible fungi varieties cultivated in my country are through natural selection. Many edible fungi have unstable properties and are prone to degeneration and aging, making it difficult to maintain the high-quality properties of these edible fungi varieties for a long time, and are more vulnerable to viral diseases. . Therefore, there is an urgent need for a new edible fungus rejuvenation and detoxification technology, which not only makes the edible fungus maintain its high-quality characters, but also restores better antiviral ability. Invention content: [0003] The present invention aims to solve the above-mentioned problems in the background technology, and provides a method for rejuvenation and detoxification of e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 范永山刘海英张运峰
Owner TANGSHAN NORMAL UNIV
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