Purification method of phloretin powder
A purification method and phloretin technology are applied in the directions of skin care preparations, separation/purification of carbonyl compounds, pharmaceutical formulations, etc., can solve the problems of difficult industrialized large-scale production and high cost, and achieve easy industrialized production, low production cost, The effect of reducing production costs
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Embodiment 1
[0023] Take 10 kg of lychee shells, put them in a multi-functional extraction tank, add 100 liters of ethanol solution with a mass concentration of 30%, heat to 100°C, stir and extract for 0.5 hours, and filter to obtain 82 liters of crude extract. The crude extract was concentrated to 8.2 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 7 liters of a concentrated solution. The concentrated solution was passed through a JK206 homogeneous strong basic anion exchange resin column, and eluted with HCl with a molar mass of 0.1M / L. The collected eluate passed through the HZ-816 macroporous non-polar adsorption resin column and was eluted with 20% ethanol. A total of 21 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to produce 40 grams of phloretin powder. The content of phloretin was 80.5% as detected by HPLC.
Embodiment 2
[0025] Take 10 kg of lychee shells, put them in a multi-functional extraction tank, add 80 liters of ethanol solution with a mass concentration of 95%, heat to 40°C, stir and extract for 4 hours, and obtain 74 liters of crude extract after filtration. The crude extract was concentrated to 15.8 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 12 liters of a concentrated solution. The concentrated solution was passed through a JK206 homogeneously porous strongly basic anion exchange resin column, and eluted with HCl with a molar mass of 0.5M / L. The collected eluate passed through the HZ-816 macroporous non-polar adsorption resin column and was eluted with 60% ethanol. A total of 23 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to prepare 60 grams of phloretin powder.
Embodiment 3
[0027] Accurately weigh about 20 mg of the extract powder in Example 2, put it in a 50 ml brown volumetric flask, add methanol, dissolve it by ultrasonic, let cool to room temperature, dilute to volume, and shake well. Accurately draw 2ml, put it in a 10ml brown volumetric flask, dilute with methanol to constant volume, pass through a 0.45um filter membrane, and inject 5ul. Detection conditions: mobile phase: acetonitrile: 0.1% phosphoric acid water = 30:70 (V:V); detection wavelength: 280nm; flow rate: 1.0ml / min; chromatographic column: C18, 250×4.6mm, 5ul. The content of phloretin was detected by HPLC to be 95.2%.
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