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Chromatography type triglyceride self-measuring system

A technology of triglycerides and triolein, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc. It can solve the problem that the blood volume cannot be measured immediately without overcoming the problem. problem, to achieve the effect of simple structure, low cost and convenient use

Active Publication Date: 2010-09-01
SUZHOU WELLBRIDGE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of kit does not require the purchase of an expensive biochemical analyzer, it still needs to be used with a microplate reader, and it does not overcome the problems of large blood consumption and inability to measure immediately

Method used

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  • Chromatography type triglyceride self-measuring system
  • Chromatography type triglyceride self-measuring system
  • Chromatography type triglyceride self-measuring system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] see Figure 1 to Figure 12 As shown, a chromatographic triglyceride self-test system includes a housing 1, a chromatography cap 2, and a reaction test paper 3 and a color test paper 4 placed in the chamber of the housing 1, corresponding to the top of the color test paper 4. The casing 1 is provided with an observation hole 5, and the upper casing 1 on both sides of the observation hole 5 is marked with a scale line (not shown). The body 1 is provided with a through hole 7 penetrating through the inner cavity of the housing 1, and the chamber of the housing 1 corresponding to the through hole 7 is provided with a liquid-inducing test paper 8; the housing 1 between the liquid-inducing test paper 8 and the reaction test paper 3 Protrude outwards to form a sampling part 9. The sampling part 9 corresponds to the upper housing 1 and has a blood sampling hole 10 through the inner cavity of the housing 1. The blood sampling hole 10 is covered with 4 layers of filter screens (n...

Embodiment 2

[0071] Such as Figure 13 As shown, a chromatographic triglyceride self-test system includes: a housing, a test paper system 26, and a solution tube 27; the housing includes an upper cover and a lower cover, and the housing is provided with a blood sampling hole 10 , a solution hole 24, an end hole 23, a receiving pad and a sliding part 25 are provided in the housing; the test paper system includes a set of liquid introduction test paper, reaction test paper and color development test paper placed in the housing chamber, the solution The system can be placed inside or outside the housing chamber; the main component of the solution contained in the solution tube is a peroxidase solution, the concentration of peroxidase is ≥0.6U / mL, and the solution system can be placed in the housing chamber Indoor or outside the housing chamber.

[0072] The blood sampling hole is covered with multiple layers of filter screens, which can filter whole blood to obtain plasma; an observation win...

Embodiment 3

[0089] Comparing the chromatographic triglyceride self-test system with the glycerol background added in the background and the chromatographic triglyceride self-test system without the addition, the results are shown in the following table and Figure 16 , Figure 17 Shown:

[0090]

[0091] The linear correlation coefficient without background is r=0.950, and the linear correlation coefficient after adding glycerin background is r=0.986. Therefore, adding background can make the measurement result more accurate.

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Abstract

The invention discloses a chromatography type triglyceride self-measuring system which comprises a shell, a set of solution-guiding test paper, reaction test paper and color-developing test paper arranged in a cavity of the shell, a solution system and a solution feeding mechanism enabling the solution system to be in contact with the solution-guiding test paper. The main component of a solution in the solution system is a peroxidase solution, and the solution system can be arranged inside or outside the cavity of the shell; the shell is provided with a blood-taking hole, a receiving pad is arranged in the shell, and the receiving pad is provided with two working positions, namely a blood-taking position and a chromatography position; lipoproteinesterase and a substrate are arranged in the receiving pad, and the substrate is selected from glycerol, glycerol trioleate, serum containing triglyceride or plasma containing triglyceride; and the reaction test paper is fixedly provided with lipoprotein lipase, phosphoglycerol oxidase, glycerokinase, adenosine triphosphate and water-soluble magnesium salt. The chromatography type triglyceride self-measuring system can realize instant measurement of triglyceride in blood.

Description

technical field [0001] The invention relates to a medical testing instrument, in particular to a chromatographic triglyceride self-test system for measuring the triglyceride content in blood and a method for using the self-test system to measure the triglyceride content in blood. Background technique [0002] Triglycerides (Triglyceride, TG) are fat molecules formed by long-chain fatty acids and glycerol. Triglyceride is the most abundant lipid in the human body. Most tissues can use triglyceride decomposition products to supply energy. At the same time, tissues such as liver and fat can also synthesize triglyceride and store it in adipose tissue. [0003] The reference range of triglycerides is: <2.3mmol / L (<200mg / dL). The level of triglycerides in normal people is affected by living conditions, and its intra-individual and inter-individual differences are greater than total cholesterol, and it gradually increases with age. Hypertriglyceridemia can be diagnosed when...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 邹丽萍顾瑜徐华良肖洁瑾
Owner SUZHOU WELLBRIDGE BIOLOGICAL TECH CO LTD
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