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Rotavirus-resistant drug acting target, building method and application method thereof

A technology of rotavirus and drug action, applied in biochemical equipment and methods, virus peptides, animal/human peptides, etc., can solve the problem of insufficient research on the mechanism of rotavirus infection, less use of anti-rotavirus, and lack of virus infection Safe and effective drugs and other issues, to achieve the effect of promoting new drug research and development

Inactive Publication Date: 2010-10-20
ARMY MEDICAL UNIV
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Problems solved by technology

[0007] So far, the research on the mechanism of rotavirus infection is still insufficient, and the clinical treatment is mainly symptomatic, and there is a lack of safe and effective drugs that directly target viral infection.
Traditional antiviral drugs gamma interferon and metabolic antiviral drugs are rarely used to fight rotavirus infection due to toxic side effects or poor efficacy

Method used

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  • Rotavirus-resistant drug acting target, building method and application method thereof
  • Rotavirus-resistant drug acting target, building method and application method thereof
  • Rotavirus-resistant drug acting target, building method and application method thereof

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Embodiment Construction

[0029] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0030] 1. Proteomics method to analyze the protein change spectrum of host cells infected by rotavirus. Use 13cm pH3-11NL IPG prefabricated gel strips for two-dimensional electrophoresis (IEF) to analyze infection with rotavirus Wa strain or SA11 strain and mock infection (mock infected) MA104 cell protein profile expression ( figure 1 A, B). Taking the simulated infection group as the control, 772 and 798 protein spots could be detected in the Wa strain infection group, and 1230 and 1312 protei...

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Abstract

The invention discloses a Rotavirus-resistant drug acting target which is a composite three-dimensional structure model of interaction of rotavirus VP6 protein and human Cyclophilin A, wherein the functional zone of the VP6 protein is amino acid from 52th to 100th site and amino acid from 349th to 392th site, and the active site comprises Asn 53, Ala 348, Val 349, Ile 355 and Pro 363; and the functional zone of the Cyclophilin A is amino acid from 54th to150th site, and the active site comprises Arg 55, Gln 63, Lys 125 and Arg 148. The invention also discloses a building method and an application method of the target; and the target and the application method thereof can simply and fast screen or design a compound capable of blocking the interaction of the rotavirus VP6 protein and the human Cyclophilin A; the invention researches and develops a specificity Rotavirus-resistant drug which is efficient and harmfulless; and the building method of the target is favor of the building of acting targets of other drugs.

Description

technical field [0001] The invention relates to a drug action target, in particular to an anti-rotavirus drug action target, a method for constructing the target, and a method for using the target to screen anti-rotavirus drugs. Background technique [0002] Rotavirus (RV) infection is the main cause of diarrhea in infants and young children, causing about 125 million cases of gastroenteritis in children every year, resulting in 352,000-592,000 deaths of children under the age of 5 due to rotavirus infection, mainly in developing countries. nation. [0003] Rotavirus belongs to the family Reoviridae and is a double-stranded RNA (dsRNA) virus without a cell membrane. Each of the 11 dsRNAs wraps 1 molecule of RNA-dependent RNA polymerase (VP1) and 1 molecule of guanylate and methyltransferase ( VP3) is located at the core of the virus particle and encodes 12 proteins, including 6 structural proteins (VP1-4, 6-7) and 6 non-structural proteins (NSP1-6). VP2 constitutes the inn...

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Application Information

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IPC IPC(8): C07K14/14C07K14/47C12Q1/70C12Q1/02G01N21/41
Inventor 李晋涛吴玉章何海洋王书峰
Owner ARMY MEDICAL UNIV
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