Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof

Abstract

The invention relates to stabilized beta-hydroxybutyric acid detection test paper and a preparation process thereof. The stabilized beta-hydroxybutyric acid detection test paper consists of a whole blood filter layer, a developing layer and a base layer which are sequentially stacked, wherein the whole blood filter layer is formed by combining at least one polymer film layer; the developing layer is made of a hydrophilic material; and the base layer is made of an insoluble supportive material. The method for preparing the stabilized beta-hydroxybutyric acid detection test paper comprises the following steps of: a, soaking the developing layer into reagent solution for 2 hours, taking the soaked developing layer out, and drying the soaked developing layer by cold air; and b, cutting the whole blood filter layer, the soaked and dried developing layer and the base layer into the same specifications, and sequentially bonding the layers with glue from top to bottom. The test paper is not limited by conditions such as region and the like; patients can conveniently perform self-monitor at any time and at any place; the concentration of beta-hydroxybutyric acid can be detected in a short time by only using micro blood; and the test paper is simple to prepare and has wide market value.

Description

Technical field:

[0001] The invention relates to a stabilized β-hydroxybutyric acid detection test paper and a manufacturing process thereof. Background technique:

[0002] Ketone bodies are special intermediates produced by the normal catabolism of fatty acids in the liver, 75% of which are β-hydroxybutyric acid. The content of β-hydroxybutyric acid in normal human blood is very small. However, under certain physiological conditions (such as starvation, fasting) or pathological conditions (such as diabetes), the source of sugar or oxidative energy supply is impaired, and fat mobilization is enhanced, and fatty acids become the main energy supply material of the human body. If the amount of ketone bodies synthesized in the liver exceeds the ability of extrahepatic tissues to utilize ketone bodies, the balance between the two will be out of balance, and the blood concentration will be too high, leading to ketoneemia and ketonuria. Beta-hydroxybutyric acid is an acidic subst...

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