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Establishing method of macaca fascicularis acute hepatic failure model

A method of establishment, technology of cynomolgus monkeys, applied in animal husbandry and other fields

Inactive Publication Date: 2010-10-27
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, none of the established animal models currently accommodate this requirement

Method used

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  • Establishing method of macaca fascicularis acute hepatic failure model
  • Establishing method of macaca fascicularis acute hepatic failure model
  • Establishing method of macaca fascicularis acute hepatic failure model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1 Materials and methods

[0031] 1.1 Material selection Adult cynomolgus monkeys, male, 7 years old, weighing 6.7kg, have not been used in other experiments, raised in single cages, with no restrictions on water and food.

[0032] 1.2 Method

[0033] 1.2.1 Preparation of D-galactosamine solution Weigh D-galactosamine hydrochloride (medicine purity is 98%) and dissolve in 5% glucose solution, the concentration of the prepared drug is 1g / 10ml, use 1mol / L The pH value of the NaOH-adjusted solution was 6.8 (pH value was measured by a digital pH meter), and the prepared solution was used within 2 hours, and was infused into the animal body through the external jugular vein catheter within 10 minutes.

[0034] 1.2.2 Establishment of the ALF model The experimental monkeys were fasted for 12 hours before the operation and could not help water. The monkey was anesthetized by Sumianxin Ⅱ (0.1mL / kg) intramuscular injection and ketamine (0.1ml / kg) intramuscular injection combined...

Embodiment 2

[0072] 1 Materials and methods

[0073] 1.1 Material selection Adult cynomolgus monkey, male, 7 years old, weighing 6.8kg, never used in other experiments, raised in a single cage, with no restrictions on water and food.

[0074] 1.2 Method

[0075] 1.2.1 The preparation of D-galactosamine solution is the same as that in Example 1.

[0076] 1.2.2 The establishment of the ALF model was the same as the procedure in Example 1, except that 20.4ml of D-galactosamine solution was infused into the experimental monkey through the external jugular vein within 10 minutes.

[0077] 1.3 Observation items

[0078] 1.3.1 Observation of survival after operation

[0079] 1.3.2 Survival time Record the survival time of animals from the time of administration

[0080] 1.3.3 Biochemical indicators Blood was drawn regularly every day before and after surgery to measure liver function, kidney function, blood ammonia, coagulation items, ratio of branched-chain amino acids and aromatic amino aci...

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Abstract

The invention relates to an establishing method of a macaca fascicularis acute liver failure model, comprising the step of injecting D-galactosamine to the external jugular vein of a macaca fascicularis. The acute hepatic failure of the macaca fascicularis is induced by an experimental mode through injecting D-galactosamine solution to the external jugular vein of the macaca fascicularis, and the established acute hepatic failure model can be well used for pre-clinical evaluation on safety and effectiveness of bioartificial liver systems.

Description

technical field [0001] The invention relates to a method for establishing an animal model of acute liver failure, in particular to a method for establishing an acute liver failure model by using cynomolgus monkeys. Background technique [0002] Acute liver failure (ALF) refers to abnormal blood coagulation and varying degrees of consciousness changes in patients without cirrhosis within 26 weeks. Currently, the only effective treatment is orthotopic liver transplantation. However, currently one-third of patients with acute liver failure die while waiting for an organ transplant due to organ shortage. As a bridge for liver transplantation or liver regeneration in patients with acute liver failure, an artificial liver support system is very necessary. [0003] The artificial liver support system has been proved by domestic and foreign scholars to have a good clinical therapeutic effect. Artificial liver support systems can be divided into non-biological artificial liver sup...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 简国登潘明新高毅张志高森周焕城
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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