Establishing method of macaca fascicularis acute hepatic failure model
A method of establishment, technology of cynomolgus monkeys, applied in animal husbandry and other fields
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Embodiment 1
[0030] 1 Materials and methods
[0031] 1.1 Material selection Adult cynomolgus monkeys, male, 7 years old, weighing 6.7kg, have not been used in other experiments, raised in single cages, with no restrictions on water and food.
[0032] 1.2 Method
[0033] 1.2.1 Preparation of D-galactosamine solution Weigh D-galactosamine hydrochloride (medicine purity is 98%) and dissolve in 5% glucose solution, the concentration of the prepared drug is 1g / 10ml, use 1mol / L The pH value of the NaOH-adjusted solution was 6.8 (pH value was measured by a digital pH meter), and the prepared solution was used within 2 hours, and was infused into the animal body through the external jugular vein catheter within 10 minutes.
[0034] 1.2.2 Establishment of the ALF model The experimental monkeys were fasted for 12 hours before the operation and could not help water. The monkey was anesthetized by Sumianxin Ⅱ (0.1mL / kg) intramuscular injection and ketamine (0.1ml / kg) intramuscular injection combined...
Embodiment 2
[0072] 1 Materials and methods
[0073] 1.1 Material selection Adult cynomolgus monkey, male, 7 years old, weighing 6.8kg, never used in other experiments, raised in a single cage, with no restrictions on water and food.
[0074] 1.2 Method
[0075] 1.2.1 The preparation of D-galactosamine solution is the same as that in Example 1.
[0076] 1.2.2 The establishment of the ALF model was the same as the procedure in Example 1, except that 20.4ml of D-galactosamine solution was infused into the experimental monkey through the external jugular vein within 10 minutes.
[0077] 1.3 Observation items
[0078] 1.3.1 Observation of survival after operation
[0079] 1.3.2 Survival time Record the survival time of animals from the time of administration
[0080] 1.3.3 Biochemical indicators Blood was drawn regularly every day before and after surgery to measure liver function, kidney function, blood ammonia, coagulation items, ratio of branched-chain amino acids and aromatic amino aci...
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