Method for preparing novel affinity medium and application thereof

An affinity and medium technology, applied in the field of biochemistry, can solve the problems of unused biochemistry, achieve good circulation, excellent performance, and the effect of ensuring health

Inactive Publication Date: 2010-10-27
北京九州泰康生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, glass plates are used as immobilization substrates for DNA and proteins in the fields of biochemistry and biochips; glass powder has the characteristics of low pri

Method used

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  • Method for preparing novel affinity medium and application thereof
  • Method for preparing novel affinity medium and application thereof
  • Method for preparing novel affinity medium and application thereof

Examples

Experimental program
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Effect test

Example

[0040] Preparation Example 1. Preparation of amino glass powder

[0041] 1. Soak the glass powder (with particle size of 0.1μm, 1μm, 10μm, 50μm, 200μm, 1000μm) in 1M NaOH for 2 hours;

[0042] 2. Soak the glass powder in ethanol with 1% 3-aminopropyltriethoxysilane and shake on a shaker at room temperature for 1 hour;

[0043] 3. Wash twice with 95% ethanol and centrifuge to dry;

[0044] 4. Vacuum drying at 150℃;

Example

[0045] Preparation Example 2

[0046] Using glass powder with a particle size of 1200 μm, amino glass powder was prepared in the same manner as in Preparation Example 1.

Example

[0047] Preparation Example 3. Preparation of aldehyde-based glass powder

[0048] 1. Put the amino glass powder prepared in the above preparation example 1 into a phosphate buffer containing 12.5% ​​glutaraldehyde, shake at room temperature for 3 hours, and wash;

[0049] 2. Vacuum drying at 150℃.

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Abstract

The invention relates to the field of biochemistry, in particular to a method for preparing a novel affinity medium and application thereof. According to the affinity medium of the invention, the grain size range of glass powder is between 0.1 and 1,000 microns. In the preparation method, the glass powder is made into a chromatography medium for separating biological substance by being subjected to surface treatment and coupling with special substances, so that the preparation method develops a brand new path for the application of the glass powder and also provides an effective and low-cost method for the separation of the biological substance. The invention provides a novel affinity medium with low cost and excellent performance. The novel affinity medium used as affinity medium directly can overcome the defect that extremely toxic substances such as cyanogen bromide are needed to be added into other media, has the advantages of environmental protection and convenient operation, and guarantees the health of scientific research personnel.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a preparation method and application of a novel affinity medium. Background technique [0002] Affinity chromatography is the most effective purification method for separating biologically active substances such as proteins. It is a chromatographic technique designed to utilize the specific affinity between biomolecules. Separation is carried out by using affinity chromatography carrier solid-phase ligands and affinity complements to combine specifically through van der Waals forces, hydrophobic forces, electrostatic forces, hydrogen bonds, and the like. This selective combination is mainly attributed to the biological characteristics and specific chemical structure and spatial conformation between the immobilized ligand and the affinity complement. In the process of affinity chromatography, the purified biomolecules selectively bind to ligands that are covalently coupled to insolubl...

Claims

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Application Information

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IPC IPC(8): B01J20/281B01J20/30
Inventor 李莹刘治平栾升
Owner 北京九州泰康生物科技有限责任公司
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