Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing novel affinity medium and application thereof

An affinity and medium technology, applied in the field of biochemistry, can solve the problems of unused biochemistry, achieve good circulation, excellent performance, and the effect of ensuring health

Inactive Publication Date: 2010-10-27
北京九州泰康生物科技有限责任公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, glass plates are used as immobilization substrates for DNA and proteins in the fields of biochemistry and biochips; glass powder has the characteristics of low price, good permeability, and difficulty in reacting with other substances, but it is only used in food, construction, industry, etc. field, not used in the field of biochemistry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing novel affinity medium and application thereof
  • Method for preparing novel affinity medium and application thereof
  • Method for preparing novel affinity medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0040] Preparation Example 1, Preparation of Amino Glass Powder

[0041] 1. Soak the glass powder (with a particle size of 0.1 μm, 1 μm, 10 μm, 50 μm, 200 μm, 1000 μm) in 1M NaOH for 2 hours;

[0042] 2. Soak the glass powder in 1% 3-aminopropyltriethoxysilane ethanol, and shake at room temperature on a shaker for 1 hour;

[0043] 3. Wash twice with 95% ethanol and dry by centrifugation;

[0044] 4. Vacuum drying at 150°C;

preparation Embodiment 2

[0046] The particle size of the glass powder is 1200 μm, and the amino glass powder is prepared in the same way as in Preparation Example 1.

preparation Embodiment 3

[0047] Preparation Example 3, preparation of aldehyde-based glass powder

[0048] 1. Put the amino glass powder prepared in the above preparation example 1 into a phosphate buffer solution containing 12.5% ​​glutaraldehyde and shake it at room temperature for 3 hours, then wash it;

[0049] 2. Vacuum drying at 150°C.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle size rangeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biochemistry, in particular to a method for preparing a novel affinity medium and application thereof. According to the affinity medium of the invention, the grain size range of glass powder is between 0.1 and 1,000 microns. In the preparation method, the glass powder is made into a chromatography medium for separating biological substance by being subjected to surface treatment and coupling with special substances, so that the preparation method develops a brand new path for the application of the glass powder and also provides an effective and low-cost method for the separation of the biological substance. The invention provides a novel affinity medium with low cost and excellent performance. The novel affinity medium used as affinity medium directly can overcome the defect that extremely toxic substances such as cyanogen bromide are needed to be added into other media, has the advantages of environmental protection and convenient operation, and guarantees the health of scientific research personnel.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a preparation method and application of a novel affinity medium. Background technique [0002] Affinity chromatography is the most effective purification method for separating biologically active substances such as proteins. It is a chromatographic technique designed to utilize the specific affinity between biomolecules. Separation is carried out by using affinity chromatography carrier solid-phase ligands and affinity complements to combine specifically through van der Waals forces, hydrophobic forces, electrostatic forces, hydrogen bonds, and the like. This selective combination is mainly attributed to the biological characteristics and specific chemical structure and spatial conformation between the immobilized ligand and the affinity complement. In the process of affinity chromatography, the purified biomolecules selectively bind to ligands that are covalently coupled to insolubl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J20/281B01J20/30
Inventor 李莹刘治平栾升
Owner 北京九州泰康生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com