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Ralstoniapickettii H2 with chlorobenzene degrability and applications thereof

A technology of Ralstonian bacteria and degrading bacteria, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., to achieve high-efficiency degradation ability, rapid degradation, and good application prospects

Active Publication Date: 2012-08-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After retrieving the relevant patent documents, there is no report on the application of Ralstonia pickettii to degrade chlorobenzene under aerobic conditions in the present invention

Method used

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  • Ralstoniapickettii H2 with chlorobenzene degrability and applications thereof
  • Ralstoniapickettii H2 with chlorobenzene degrability and applications thereof
  • Ralstoniapickettii H2 with chlorobenzene degrability and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Isolation, purification and identification of Ralstonia pickettii strainH2

[0047] 1. Separation and purification of Ralstonia pickettii strainH2

[0048] Ralstoniapickettii strainH2 is a gram-negative bacterium obtained by collecting the biofilm on the surface of a biotrickling filter bed filler for purifying chlorobenzene waste gas, and separating and purifying it. Specific steps are as follows:

[0049] Take two fillers from the biotrickling filter bed, wash the biofilm in a shaker flask with 30mL sterile water, place on a shaker at 160rpm, and shake at 30°C for 1 hour. Then use the chlorobenzene-degrading bacteria screening medium for strain screening. Chlorobenzene-degrading bacteria screening medium composition: KH 2 PO 4 , 0.5g; Na 2 HPO 4 .12H 2 O, 0.5g; (NH 4 ) 2 SO 4 , 2g; MgSO 4 ·6H 2 O, 0.1g; CaCl 2 , 0.01g; MnSO 4 ·H 2 O, 0.13 mg; ZnCl 2 , 0.23mg; CuSO 4 ·H 2 O, 0.03 mg; CoCl 2 ·6H 2 O, 0.42 mg; Na 2 MoO 4 2H 2 O, 0.15 mg;...

Embodiment 2

[0058] Example 2: Detection of Ralstonia pickettii H2 biodegradability of p-chlorobenzene

[0059] 1. Biodegradation characteristics of p-chlorobenzene by bacteria Ralstonia pickettii H2 at different temperatures

[0060] The degradation experiment of Ralstonia pickettii H2 p-chlorobenzene was carried out at different temperatures, and it was found that it has a high ability to degrade benzene compounds at 25-38°C. From the perspective of practical application, 30°C is the best temperature, and the removal rate at this time The highest, the specific implementation steps are as follows:

[0061] Take 150mL inorganic salt culture medium (KH 2 PO 4 , 0.5g; Na 2 HPO 4 .12H 2 O, 0.5g; (NH 4 ) 2 SO 4 , 2g; MgSO 4 ·7H 2 O, 0.1g; CaCl 2 , 0.01g, MnSO 4 ·H 2 O, 0.13 mg; ZnCl 2 , 0.23mg; CuSO 4 ·H 2 O, 0.03 mg; CoCl 2 ·6H 2 O, 0.42 mg; Na 2 MoO 4 2H 2 O, 0.15 mg; AlCl 3 ·6H 2 O, 0.05mg; distilled water to make up to 1000mL) was divided into 14 500mL anaerobic bott...

Embodiment 3

[0076] Embodiment 3: Ralstonia pickettii H2 detects the biodegradability of benzene, dichlorobenzene, phenol, chlorophenol

[0077] Take 150mL inorganic salt culture medium and divide into eight 500mL anaerobic bottles, and sterilize. Divide into 2 groups, 4 bottles in each group, add freshly cultivated H2 bacterial suspension to each bottle in one group, OD 600 0.15, the inoculum size was 2mL / 150mL, and the only carbon source was benzene / dichlorobenzene / phenol / chlorophenol with an initial concentration of 50mg / L. They were respectively placed in a shaker at 30°C at 160rpm for cultivation; another 4 bottles of the same uninoculated culture medium were respectively placed at the corresponding temperature for simultaneous cultivation as a blank control. After cultivating for 36 hours, samples were taken to detect the concentration of benzene / dichlorobenzene by gas chromatography, and the concentration of phenol / chlorophenol by liquid chromatography. The results were as follows:...

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Abstract

The invention provides chlorobenzene degrading bacteria, namely Ralstoniapickettii H2 which can high efficiently degrade chlorobenzene and derivative thereof. The Ralstoniapickettii H2 is preserved in the China Center for Type Culture Collection (CCTCC); the preservation data is Nov.4, 2009; the preservation number is CCTCC No: M 209250. The invention has the effects of providing a strain which can high efficiently and rapidly degrade chlorobenzene compound; the strain has certain broad spectrum in degrading pollutants, has higher efficient degrading capacity to chlorobenzene, has certain broad spectrum in degrading chlorine aromatic compound and derivative thereof, and has good application prospect in biological purification of industrial waste gas and wastewater.

Description

(1) Technical field [0001] The invention relates to a chlorobenzene-degrading bacterium capable of efficiently degrading chlorobenzene and its derivatives—Ralstonia picoformis H2 and its application. (2) Background technology [0002] Chlorobenzene is a volatile monocyclic halogenated aromatic compound, widely used in dyes, plastics, spices, medicines, pesticides, organic synthesis intermediates; for the manufacture of phenol, nitrochlorobenzene, aniline and pesticides DDT; It is also used to prepare solvents and rubber additives, paints, quick-drying inks and dry cleaning agents, etc. It is a kind of toxic organic compound that is widely distributed in the environment and difficult to biodegrade. Chlorobenzene compounds are bioaccumulative, biotoxic, and carcinogenic to humans, and are included in the list of priority pollutants by the U.S. Environmental Protection Agency (EPA). Biodegradation technology has the characteristics of mild reaction conditions, low operating co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/22
Inventor 张丽丽陈建孟朱润晔冷守琴王家德蒋轶锋
Owner ZHEJIANG UNIV OF TECH
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