1% osmium tetroxide fixative and preparation method thereof

Abstract

The invention discloses a 1% osmium tetroxide fixative and a preparation method thereof. The following materials are adopted in the method which comprises the following steps of: (1) preparing a phosphate buffer solution from a disodium hydrogen phosphate solution and a sodium dihydrogen phosphate solution, and carrying out intermediate product inspection after the preparation, wherein the preparation process can be checked by two persons; (2) adding the phosphate buffer solution prepared in the step (1) to osmium tetroxide and distilled water, then purifying and carrying out pH value inspection; and (3) carrying out fused subpackaging on the solution prepared in the step (2) with brown ampoules, carrying out final examination after packaging, and then delivering out of warehouse. The invention has the advantages of high quality, experiment time saving, experiment cost saving, long quality guarantee period and the like, reduces the pollution of outdated reagent to the environment and prevents reagent preparation from doing harm to the health of experimenters.

Description

Technical field: [0001] The invention relates to a chemical reagent product, in particular to a 1% osmium tetroxide fixative used in bioelectron microscope experiments and a preparation method thereof. Background technique: [0002] The 1% osmium tetroxide fixative used in electron microscopy experiments is mostly prepared by the laboratory. The reagents are often made of osmium tetroxide crystals and 1% osmium tetroxide is prepared with phosphate buffer solution; the amount of reagents used in electron microscopy experiments is generally very small , 1mL of 1% osmium tetroxide fixative can process up to 8 samples, and the minimum packaging of raw materials purchased by self-made reagents: 0.5g of osmium tetroxide, 500g of sodium dihydrogen phosphate, 500g of disodium phosphine, and 10 samples Calculated, the remaining reagent in the experiment is 98.9%; in addition, osmium tetroxide is very expensive, the price of 0.5g / piece: 580 yuan, and this reagent needs to be prepared ...

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Problems solved by technology

[0002] The 1% osmium tetroxide fixative used in electron microscopy experiments is mostly prepared by the laboratory. The reagents are often made of osmium tetroxide crystals and 1% osmium tetroxide is prepared with phosphate buffer solution; the amount of reagents used in electron microscopy experiments is generally very small , 1mL of 1% osmium tetroxide fixative can process up to 8 samples, and the minimum packaging of raw materials purchased by self-made reagents: 0.5g of osmium tetroxide, 500g of sodium dihydrogen phosphate, 500g of disodium phosphine, and 10 samples Calculated, the remaining reagent in the experiment is 98.9%; in addition, osmium tetroxide is very expensive, the price of 0.5g / piece: 580 yuan, and this reagent needs to be prepared at one time, and the prepared 1% osmium tetroxide fixative has strong reduction nature, the placement process will quickly fail, and will eventually be abandoned, which not only wastes the experimental funds of the experimenters, but also wastes resources. The abandoned reagents are highly toxic and require special treatment, which is likely to cause environmental pollution; It takes about 2 days for the reagent to be cleaned from the container, dried, weighed, and prepared; osmium tetroxide is a highly toxic chemical, and its toxicity will also affect the health of the preparer

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