Enzyme-linked immunosorbent assay method for egg allergen ovalbumin in foods

An enzyme-linked immunoassay and ovalbumin technology, applied in the field of immune analysis, can solve the problems of high cost, restricting the development of allergen detection methods, and slow detection speed, and achieve low detection cost, simple pretreatment process, and less time-consuming Effect

Active Publication Date: 2013-05-15
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods for allergen detection at present, they all have different problems, such as slow detection speed, high cost, and the need for specific analytical instruments, etc., which restrict the development of allergen detection methods in food

Method used

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  • Enzyme-linked immunosorbent assay method for egg allergen ovalbumin in foods

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] specific implementation plan

[0026] The present invention is further illustrated by the following examples.

[0027] 1. Instrument:

[0028] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory

[0029] KFLOW pure water machine, KFLOW company

[0030] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory

[0031] Costar 96-well 8×12 detachable microtiter plate, Shanghai Jitai Biotechnology Co., Ltd.

[0032] Multiska Mks microplate reader, Thermo Labsystems

[0033] Adjustable pipette, Thermo Labsystems

[0034] Vortex mixer, Shanghai Huxi Instrument Analysis Factory

[0035] 2. Reagents:

[0036] Horseradish peroxidase-labeled goat anti-rabbit IgG (HRP-IgG), Kangcheng Bioengineering Company Tetramethylbenzidine (TMB), Huamei Bioengineering Company

[0037] Other reagents are analytical reagents

[0038] 3. Steps

[0039] 1. Preparation of antiserum (polyclonal antibody)

[0040] 1) Experimental animals: 2...

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Abstract

The invention discloses an Enzyme-linked immunosorbent assay (ELISA) method for egg allergen ovalbumin in foods, which belongs to the technical field of immunoassay. In the invention, a polyclonal antibody is obtained by immunizing a healthy New Zealand white rabbit with high-purity ovalbumin, and an indirect ELISA method for ovalbumin in foods is established by using the antibody as a detecting agent and the high-purity ovalbumin as a standard product and a coating antigen. In the invention, a quick and high-efficiency detection means is provided for detecting the ovalbumin content of the foods is provided; due to the adoption of the polyclonal antibody, the cost is low and the stability and the repeatability are high; the sensitivity is 1ppm, the linear range is 1 to 128ppm; and the high specificity and affinity of an immunoreactions ensure that the ELISA has extremely high selectivity and sensitivity.

Description

technical field [0001] The invention relates to an enzyme-linked immunoassay method for quantitatively detecting the content of egg allergen ovalbumin in food, and belongs to the technical field of immune analysis. Background technique [0002] In recent years, food allergy has become a public food safety problem, and eggs are one of the eight most common food allergens. Allergic diseases seriously endanger people's health. Although the minimum amount of allergens that trigger allergic reactions varies with different groups of people, a small amount of allergens can cause allergic symptoms in most patients. In order to avoid exposure to trace amounts of allergens, the detection of allergens has become a top priority. Now, although there are many allergen detection methods for reference, there are different problems in specific applications. The in vivo test method can provide the most direct evidence, but due to safety factors and other considerations, it can only be carri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/558G01N21/31
Inventor 胥传来邓小芳勇倩倩马文蔚陈莲君严文静屈昌龙吴晓玲
Owner JIANGNAN UNIV
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