Foot and mouth disease virus-like particle, preparation method and application thereof

A technology of foot-and-mouth disease virus and particles, applied in botany equipment and methods, biochemical equipment and methods, antiviral agents, etc., can solve the problems of limiting the contribution of animal husbandry to the healthy development of the national economy, affecting the export of animals and their products, etc. To achieve the effect of overcoming low immune efficiency, good antigenicity or immunogenicity, and high expression efficiency

Active Publication Date: 2010-12-15
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevention and control of inactivated vaccines currently used can no longer adapt to the current international trade situation, which has greatly affected the export of animals and their products in my country, and severely limited the contribution of animal husbandry to the healthy development of animal husbandry and the national economy.

Method used

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  • Foot and mouth disease virus-like particle, preparation method and application thereof
  • Foot and mouth disease virus-like particle, preparation method and application thereof
  • Foot and mouth disease virus-like particle, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] 1. Construction of small ubiquitin-like modified protein fusion expression vector:

[0037] Primers were designed according to the published Saccharomyces cerevisiae smt3 gene (GenBank accession number: AY558174), and the primer sequences are shown in SEQ 5 and SEQ 6 in the gene sequence table.

[0038] In the upstream primer smt3F (5'G CCATGG (Nco I) GT CAT CAC CAT CAT CAT CAC (6×His) GGG TCG GAC TCAGAA GTC AAT CAA 3') Introduce Nco I endonuclease site and 6×His coding sequence, in the downstream primer smt3R (5' GGA TCC (BamH I) GAGACC (Bsa I) TTAA GGTCTC (Bsa I)AA CCT CCA ATC TGT TCG CGG TG 3') introduces a sequence comprising a BamH I endonuclease site and a Bsa I endonuclease site in each of the positive and negative duplexes. Referring to the method of Motejadded et al. (2009) (Motejadded H, Altenbuchner J, 2009. Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing. Biotechnol Lett. 31 (4):...

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Abstract

The invention discloses an Asia I type foot and mouth disease virus-like particle, a preparation method and an application thereof. The Asian I type foot and mouth disease virus-like particle comprises structural proteins of VP0, VP3 and VP1 of Asia I type foot and mouth disease virus, wherein the gene sequence of VP0 is shown in SEQ 1, the gene sequence of VP3 is shown in SEQ 3, and the gene sequence of VP1 is shown in SEQ 2. The preparation method of the Asian I type foot and mouth disease virus-like particle has the following steps: performing amplification to obtain the VP0, VP3 and VP1, performing enzyme digestion to obtain a recombinant expression vector, performing enzyme digestion on fusion protein by small ubiquitin-like modifier (SUMO), and carrying out in-vitro assembling to obtain the foot and mouth disease virus-like particle.

Description

technical field [0001] The invention relates to an Asian type I foot-and-mouth disease virus-like particle, a preparation method and application of the virus-like particle. Background technique [0002] Foot-and-mouth disease is the most serious disease that harms pigs, cattle, and sheep, the main livestock breeds in my country. The value creation of these livestock breeds accounts for about 80% of the output value of animal husbandry. The economic loss caused by the disease reaches tens of billions of yuan every year, and it also involves food safety and public health. my country attaches great importance to the prevention and control of this disease, which is of great significance to promote the adjustment of agricultural industrial structure and accelerate the development of "three rural areas". [0003] After joining WTO, developed countries restricted the export of my country's live animals and their products through technical barriers to animal husbandry products, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04C12N15/42C12N15/63A61K39/135A61P31/14
Inventor 孙世琪郭慧琛杨顺利沈小燕尹双辉尚佑军刘湘涛殷宏才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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