Foot and mouth disease virus-like particle, preparation method and application thereof

Abstract

The invention discloses an Asia I type foot and mouth disease virus-like particle, a preparation method and an application thereof. The Asian I type foot and mouth disease virus-like particle comprises structural proteins of VP0, VP3 and VP1 of Asia I type foot and mouth disease virus, wherein the gene sequence of VP0 is shown in SEQ 1, the gene sequence of VP3 is shown in SEQ 3, and the gene sequence of VP1 is shown in SEQ 2. The preparation method of the Asian I type foot and mouth disease virus-like particle has the following steps: performing amplification to obtain the VP0, VP3 and VP1, performing enzyme digestion to obtain a recombinant expression vector, performing enzyme digestion on fusion protein by small ubiquitin-like modifier (SUMO), and carrying out in-vitro assembling to obtain the foot and mouth disease virus-like particle.

Description

technical field [0001] The invention relates to an Asian type I foot-and-mouth disease virus-like particle, a preparation method and application of the virus-like particle. Background technique [0002] Foot-and-mouth disease is the most serious disease that harms pigs, cattle, and sheep, the main livestock breeds in my country. The value creation of these livestock breeds accounts for about 80% of the output value of animal husbandry. The economic loss caused by the disease reaches tens of billions of yuan every year, and it also involves food safety and public health. my country attaches great importance to the prevention and control of this disease, which is of great significance to promote the adjustment of agricultural industrial structure and accelerate the development of "three rural areas". [0003] After joining WTO, developed countries restricted the export of my country's live animals and their products through technical barriers to animal husbandry products, and...

AI Technical Summary

Problems solved by technology

The prevention and control of inactivated vaccines currently used can no longer adapt to the current international trade situation, which has greatly affected the export of animals and their products in my country, and severely limited the contribution of animal husbandry to the healthy development of animal husbandry and the national economy.

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