Method for measuring bicarbonate ion utilizing capability of plant

Abstract

The invention discloses a method for measuring the bicarbonate ion utilizing capability of a plant. The method comprises the following steps of: (1) respectively measuring stable carbon isotope compositions, i.e. delta 13C values of leaves of a plant to be studied and a reference plant; (2) measuring the stable carbon isotope composition, i.e. delta 13C value of microalgae; (3) substituting the delta 13C value of the leaves of the plant to be studied, the delta 13C value of the leaves of the reference plant and the delta 13C value of the microalgae into a two-end-member model and calculating the proportional share of bicarbonate ions utilized by the plant to be studied in an inorganic carbon source; (4) measuring the net photosynthetic rate of the leaves of the plant to be studied; and (5) deriving the bicarbonate ion utilizing capability of the plant to be studied according to the net photosynthetic rate of the leaves of the plant to be studied and the proportional share of the bicarbonate ions utilized by the plant to be studied in the inorganic carbon source. The invention can be used for quantitatively measuring the bicarbonate ion utilizing capability of the plant with less plant materials and less steps and simple calculation.

Description

technical field [0001] The invention relates to a method for measuring the ability of plants to utilize bicarbonate ions, and belongs to the field of ecological environment management. Background technique [0002] Plants can not only use carbon dioxide in the air as a raw material for photosynthesis, but also use stored bicarbonate ions as a raw material for photosynthesis through the action of carbonic anhydrase. For karst regions, the ability of plants to utilize bicarbonate ions is particularly important. After karst plants are subjected to karst adversity (karst drought, high calcium, pH, bicarbonate ions, and low inorganic nutrients, etc.), the activity of carbonic anhydrase in leaves increases, which leads to the decrease or closure of stomatal conductance on the one hand. Reduces transpiration to prevent further dehydration of plants, on the other hand converts intracellular bicarbonate ions into water and CO 2 , to deal with moisture and CO caused by stomatal cond...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for measuring the ability of plants to utilize bicarbonate ions, so as to overcome the deficiency in the prior art that plant leaves cannot be used for photosynthesis using bicarbonate ions

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