A kind of method for measuring the tolerance of plants to sub-low temperature or sub-high temperature

A sub-low temperature and sub-high temperature technology, applied in the field of plant temperature tolerance measurement, can solve the problems of inability to effectively distinguish physiological activity and decrease of physiological activity, and achieve the effect of novel technical principle, rapid method and universal applicability
CN104830947BInactive Publication Date: 2017-06-23HAINAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HAINAN UNIV
Publication Date
2017-06-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for measuring the sub-low temperature or sub-high temperature tolerance of plants, which comprises the following steps: (1) selecting tender plant tissues, after crushing, adding enzymatic hydrolysis solution and mixing them evenly, and enzymolyzing their cell walls; (2) After the product is washed and purified, a protoplast suspension is obtained, and hydrolytic enzymes are added to the protoplast suspension to maintain low-level regeneration of cell wall cellulose; Warm bath, after the warm bath is over, add ultrapure water to reduce the osmotic potential of the solution, and regularly measure the diameter of the protoplast; (4) use the obtained diameter value of the protoplast to calculate the unit time, unit osmotic potential, and cell membrane per unit area of ​​multiple protoplasts The average value of the net velocity Lp of water entering the cell from the outside of the cell, the greater the difference between the average value of Lp and the Lp value at the appropriate temperature of the selected plant, the worse its sub-low temperature or sub-high temperature tolerance. This method has the characteristics of wide applicability and high resolution.
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Description

technical field

[0001] The invention belongs to the technical field of measuring plant temperature tolerance, in particular to a method for measuring the tolerance of plants to sub-low temperature or sub-high temperature. Background technique

[0002] Temperature is the most important environmental factor for plant growth. High temperature or low temperature will cause the growth and development of plants to be hindered or even damaged.

[0003] Different plants have different temperature requirements, and even the same plant has different temperature requirements in different growth stages. The so-called sub-low temperature or sub-high temperature refers to the temperature condition that only slows down the growth of crops but does not cause major damage to the crops and cause their growth to stagnate.

[0004] The tolerance of plants to sub-low temperature and sub-high temperature is called sub-low temperature tolerance or sub-high temperature tolerance. different from e...

Claims

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