Swing-driven circular cell culture device

Abstract

The invention relates to a swing-driven circular cell culture device. The culture device comprises a hard culture container the lower part of which is provided with a solution chamber; the bottom surface of the solution chamber is a smooth concave surface; a slope surface is arranged on the side wall surface of the solution chamber; the slope surface and the concave surface are in smooth transition; a circular pipe is arranged on the side wall of the solution chamber along the slope surface in a matched manner; the circular pipe is provided with an upper opening and a lower opening; and a one-way discharge valve structure is arranged at the lower opening. When the circular cell culture device is driven to swing left and right, the upper culture solution constantly enters from the upper openings of the two circular pipes and rushes out from the lower openings, while the original lower culture solution is turned upwards in the process to realize up-down circulation of the culture solution; the circulation is simple and effective, and the mixing and ventilation effect is good; and moreover, the circulation frequency can be minimized and the shearing force of the circulation is little.

Description

technical field [0001] The present invention relates to a cell culture device in which a culture liquid circulates when a rocking drive is applied to it. Background technique [0002] The cultivation of organisms (including the cultivation of animal cells, plant cells, insect cells and microorganisms) and the use of the above-mentioned cultured organisms for biotechnology products (such as proteins, polypeptides, nucleic acids, antibodies, virus vectors and virus vaccines) ) production, it is necessary to provide appropriate temperature, necessary gases (such as oxygen and carbon dioxide gas) and proper mixing, so there are various products on the market that are suitable for it, such as incubators, carbon dioxide incubators, mixing shakers , fermenters, culture bottles and various cell bioreactors, etc. When performing cell culture, especially animal cell culture and the use of animal cells for the production of the above-mentioned biotechnology products, precise temperatu...

AI Technical Summary

Problems solved by technology

[0003] At present, the biological culture devices used for animal cell culture on the market mainly include spinner bottles, magnetic stirring culture flasks, NBS basket static cell culture bioreactors and WAVE rocking bag bioreactors, of which the spinner bottles and magnetic stirring culture flasks are only used Suitable for small-scale production and step-by-step scale-up, due to its non-disposable use, it is easy to cross-contaminate

In addition, magnetic stirring culture has the disadvantage of high shear force.

NBS basket static cell culture bioreactor is expensive and complicated to operate, and is not suitable for small-scale cultivation in the laboratory, and its non-disposable culture tank has cross-contamination problems

WAVE rocking bag bioreactor is to culture cells in disposable plastic soft bags. Waves are generated by shaking bags to suspend cells, mix culture medium, and exchange gases. The shear force is relatively small, but it has the following disadvantages : First of all, WAVE is mainly suitable for large-scale cell culture. The patent of WAVE shows that the culture volume should be above 5L. When the culture volume is small, it cannot generate enough waves to suspend the cells, and the cells are deposited at the bottom of the bag. During the shaking process Mutual friction and damage; secondly, the WAVE shaker bag is a plastic soft bag, coupled with a series of connected pipes, the operation is more complicated than the hard body culture container; thirdly, different types of WAVE culture bags need to use the matching shaker, the small bag part A large shaker can be used, but a small shaker cannot be used for large bags. A series of expensive equipment needs to be purchased for small-scale cultivation and scale-up to large-scale industrial production; finally, although WAVE has less shear force than agitated bioreactors, However, to produce the necessary cell suspension, sufficient mixing and gas exchange, a certain rocking speed and frequency are also required, so a certain degree of shear force will also be generated.

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