40 results about "Cell culture bioreactor" patented technology

An apparatus and method for a modular cell culture bioreactor comprises a plurality of chambers for cell culture; at least one reservoir containing a cell support medium; a plurality of conduits fluidly connecting the at least one reservoir with the plurality of chambers; and at least one pump fluidly connected through the plurality of conduits with the at least one reservoir and with the plurality of chambers to pump cell support medium therethrough; wherein each individual chamber of the plurality of chambers includes at least one three-dimensional matrix comprising polyethylene terephthalate, a plurality of channels carrying the cell support medium and having the matrix positioned in fluid communication therebetween, and at least two openings into each the channel, wherein a first the opening is in fluid connection with the pump and a second the opening is in fluid connection with the reservoir.

The invention provides a disposable cell culture bioreactor manifold system for use in coupling sensors, fluid samplers, conduits, and the like, to a cell culture bioreactor in a sterile manner. The disposable bioreactor manifold system includes an externally attachable bioreactor manifold connector body for fluidly attaching modular sensor arrangements that measure physical variables and other parameters of medium contained within a bioreactor, as well as medium sampling components, and other connections, as well as at least one conduit fluidly connecting connecting the bioreactor manifold connector body with a pump for pumping fluids between the bioreactor and the bioreactor manifold connector body.

Methods and devices for cell or tissue culture are provided. One aspect provides a bioreactor having a gas permeable, closed-loop chamber for cell or tissue culture, and an oscillating means for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force convection of cells and fluid in the gas permeable, closed-loop chamber. The bioreactor optionally includes a tissue engineering scaffold, an inlet means, an outlet means, and integrated sensors. Another aspect provides a bioreactor having a plurality of gas permeable, closed-loop chambers for cell or tissue culture. Methods of culturing cells and producing tissue constructs are also provided.

A cell culture bioreactor having a culture chamber for cell growth, a culture medium reservoir mounted externally to the culture chamber, a fluid circulation mechanism for moving culture medium from the reservoir through the culture chamber, and an aerating mechanism for introducing air into and removing air from the culture chamber is provided. The cell culture bioreactor comprises a manifold mechanism mounted at the top end of the culture chamber, a fluid circulation mechanism fluidly connected to the culture medium reservoir, for receiving culture medium from the culture medium reservoir and distributing culture medium into the culture chamber. A support matrix is mounted within the culture chamber in fluid communication with the fluid circulation mechanism with at least a portion of the support matrix contacting the culture medium wherein the culture medium flows from the manifold means in a thin film over substantially the entire surface of the support matrix allowing the biological cells to grow on the support matrix.

A single-use bag includes a collapsible a body having a first end and an opposing second end bounding a compartment. The collapsible body comprises at least one flexible sheet having an interior surface and an exterior surface; wherein the interior surface layer comprises low density polyethylene having a melt index from 0.9 to 2.0 g/10 min with a weight average molecular weight (Mw) greater than 200,000 Da, and an Mz greater than 1,500,000 Da. Optionally, the flexible sheet comprises an oriented film as an additional layer on the exterior surface. The single-use bag is useful in biological processing, including use in a disposable cell culture bioreactor.

The invention relates to a sampling device suitable for bioreactors such as microbial fermentation and cell culture, in particular to a concentration gradient diffusion sampling device, which is based on the concentration difference caused by the concentration difference of each component in the liquid in the solution on both sides of the separation membrane. The principle is to form a concentration gradient distribution of components (measured samples) in the sampling pipeline, and the flux or rate of diffusion follows Fick's law. Using this device to sample the samples in the bioreactor has the advantages of effectively preventing sampling pollution and good safety; there is no filtration process of the solution, the membrane pores should not be blocked, and the stability of continuous sampling is high; the sample components are in the sampling pipeline A gradient distribution is formed, which can be measured without cumbersome dilution, making the online detection system simpler, with faster measurement speed and wider linear range, and is especially suitable for on-line detection of various flow injection analysis systems.

The present invention relates to a scaleable bioreactor comprising at least one cassette comprising a manifold and an arrangement of hollow fibre membranes; an upper headplate; and a lower headplate, wherein the cassette(s) are modular components adapted to co-operate with each other and the headplates to define an internal extracapillary culture space (ECS) and wherein the arrangement of hollow fibre membranes includes a discrete inlet and outlet. The invention further relates to a kit for such a bioreactor, a cassette for use in such a bioreactor and a process for exploiting the metabolism of cells and/or microorganisms, the process including the step of utilising a bioreactor according to the invention.

The invention relates to an external circulation animal cell culture bioreactor which comprises a tank body, an external circulation pipeline, a heater, a peristaltic pump and a control system, wherein the tank body comprises a large tank and a small tank; the small tank is sheathed in the large tank and is clamped and fixed with the large tank respectively through ducts D, A and G; the external circulation pipeline comprises a tank external circulation pipeline and ducts A, B, E and D; on the upper part of the outside of the tank body, the ducts A and B are connected with the tank external circulation pipeline through a tee joint; on the lower part of the outside of the tank body, the ducts E and D are connected with the tank external circulation pipeline through a tee joint; the tank external circulation pipeline penetrates through the peristaltic pump; and the lowest position of the duct D is connected with a drainage pipe. The invention fully displays the characteristics of the animal cell large-scale culture technique, has the characteristics of thorough sterilization, simple operation, low pollution rate of cell culture, good quality and high yield, has favorable functionality and economical efficiency, and can be widely used for animal cell microcarrier suspension culture and animal cell suspension culture.

The invention relates to a bioreactor system characterized by its capacity in cultivating cells in all three states: static, dynamic, or alternating between static and dynamic states in the same cell culture container or containers, with the even distribution of cells in cell static culture following a dynamic culture. In the invented bioreactor system, the combined application of the magnetically controlled agitation and the cell culture container inversion as well as the combined application of the vertical rotating culture and horizontal static culture are the two strategies in building ideal bioreactors for the cell culture alternating between static and dynamic states in the same cell culture container, which can minimize the sheer-stress and provide cells an ideal metabolic environment.

The invention relates to a macrophage medium and a culture method. The macrophage medium contains a DMEM medium, FBS, GM-CSF, lipopolysaccharide and interleukin-14. In the culture method of macrophage, the macrophage is cultured by use of the macrophage medium provided by the invention, a microcarrier and a shaking bag type cell culture bioreactor. By adopting the macrophage medium and culture method provided by the invention, the culture time is shortened, the consumption of human resources is reduced, and large-scale culture of macrophage can be realized.

The invention provides a cell culture bioreactor and belongs to the technical field of cell culture. The cell culture bioreactor comprises a tank body, wherein an upper air inlet is formed in the upper part of the tank body, and a lower air inlet is formed in the lower part of the tank body; a magnetic stirring mechanism is arranged in the bottom center of the tank body; the magnetic stirring mechanism is connected with a stirring shaft in the tank body; an upper blade is arranged at the top end of the stirring shaft; a first stirring mechanism and a second stirring mechanism in alternative distribution are sequentially arranged on the stirring shaft from top to bottom; the second stirring mechanism is longer than the first stirring mechanism; a microbubble ring is arranged below the stirring shaft; the microbubble ring comprises at least two hollow sub-rings with diameter gradually reducing from top to bottom and a first air inlet rod connecting the sub-rings; the first air inlet rodis communicated with the lower air inlet by a second air inlet rod; multiple air holes in uniform distribution are formed in the lower surface of the sub-rings. The cell culture bioreactor provided bythe invention has the advantages of uniform ventilation, high efficiency, uniform mixing of cells and nutrient substances, high yield and uniform product quality while meeting the need for large-volume tank production.

The invention relates to a cell culture biological production device, mainly aiming at solving the problem that when the cell culture biological reaction device in the prior art is used for carrying out cell culture, the maximum cell density in the bioreactor is low. The cell culture biological production device comprises a cell culture bioreactor and a culture solution oxygenation tower, wherein the inside of the oxygenation tower has the following arrangement: the upper space is provided with a liquid distributor, the lower space is provided with an oxygenation tower gas distributor communicated with an oxygenation gas inlet, and a filler layer is arranged between the liquid distributor and the oxygenation tower gas distributor; an oxygenation tower tail gas discharge hole is formed in the top of the oxygenation tower; the production device is provided with an oxygenation tower feeding channel I used for taking a liquid material from the reactor and conveying the liquid material to the top of the oxygenation tower and communicated with the liquid distributor; and the production device is provided with a liquid material back conveying channel II used for taking the liquid material from the bottom of the oxygenation tower and conveying the liquid material to the reactor.

A schizothorax quadricarinatus fin ray cell culture bioreactor comprises a base, vertical supporting plates are fixedly installed on the two sides of the base respectively, a motor is fixedly installed in the center of the base, a sliding block with a dovetail-shaped section is arranged in a sliding groove in a matched mode, the bottom end of the sliding block is hinged to the top end of the otherside of a connecting plate, and an air inlet is formed in one side of a sleeve. A pipeline is fixedly connected to the top end of the sleeve and communicated with the interior of the sleeve, and theother end of the pipeline is fixedly connected with the top end of the reactor body through a sealing bearing and communicated with the interior of the reactor body. According to the present invention, the shaking rotation can be performed, the shearing force generated by the machine is reduced, the influence of the mechanical stirring on the cells is avoided, the damage or the infection of the cells caused by the mechanical stirring is avoided, other devices are not required to provide power for the oxygen supply, the oxygen supply is performed through the movement of the reactor body, and the power device is less; better effect.