External circulation animal cell culture bioreactor

Abstract

The invention relates to an external circulation animal cell culture bioreactor which comprises a tank body, an external circulation pipeline, a heater, a peristaltic pump and a control system, wherein the tank body comprises a large tank and a small tank; the small tank is sheathed in the large tank and is clamped and fixed with the large tank respectively through ducts D, A and G; the external circulation pipeline comprises a tank external circulation pipeline and ducts A, B, E and D; on the upper part of the outside of the tank body, the ducts A and B are connected with the tank external circulation pipeline through a tee joint; on the lower part of the outside of the tank body, the ducts E and D are connected with the tank external circulation pipeline through a tee joint; the tank external circulation pipeline penetrates through the peristaltic pump; and the lowest position of the duct D is connected with a drainage pipe. The invention fully displays the characteristics of the animal cell large-scale culture technique, has the characteristics of thorough sterilization, simple operation, low pollution rate of cell culture, good quality and high yield, has favorable functionality and economical efficiency, and can be widely used for animal cell microcarrier suspension culture and animal cell suspension culture.

Description

technical field [0001] The invention relates to a device for cultivating animal cells by adopting a suspension method, in particular to an external circulation animal cell culturing bioreactor. Background technique [0002] Bioreactor refers to equipment that uses living cells or enzymes as biocatalysts to provide a suitable environment for cell proliferation or biochemical reactions. It is the key equipment in the process of biological reactions. The selection of the structure, operation mode and operating conditions of the bioreactor is closely related to the quality, yield (conversion rate) and energy consumption of biochemical products. [0003] With the continuous development of biotechnology, large-scale animal cell bioreactor culture technology is gradually replacing glass bottle static culture and rotary culture technology, because it can provide the best living environment for cells and viruses in time, so that the most efficient It should be said that this is a qu...

AI Technical Summary

Problems solved by technology

[0003] With the continuous development of biotechnology, large-scale animal cell bioreactor culture technology is gradually replacing glass bottle static culture and rotary culture technology, because it can provide the best living environment for cells and viruses in time, so that the most efficient It should be said that this is a qualitative leap in the history of animal cell culture and a major breakthrough in biopharmaceutical technology. It not only simplifies the process, but more importantly, it can produce high-quality products. There are many kinds of bioreactors on the market, including domestic and imported ones, each has its own advantages and disadvantages, and some even have unavoidable flaws, which limit the maximum use of its advantages

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