Cell culture biological reactor

Abstract

The invention discloses a cell culture biological reactor, comprising a big tank, a small tank and a control system; the small tank is arranged in the big tank and supported by a supporting frame at the bottom part of the big tank; the upper end of the small tank is provided with a seal end, and the lower end is provided with a magnetic stirring system; the bottom part closing to the tank bottom position is provided with an isolating plate, and the seal end and the isolating plate are stainless steel mesh plates; a fluid feeding pipe, a fluid discharging pipe, a sensor system, gas inlet and outlet pipes are further arranged in the big tank and the small tank; several clamping devices are further arranged on the tank body of the small tank. Therefore, many forms of cell culture can be realized by one reactor, and the reactor is featured by good sealing property, simple operation, low pollution rate, good cell quality, and high yield. Besides, the reactor has good functionality and economic efficiency, and is widely applied to the large-scale culture of animal cells.

Description

technical field [0001] The invention belongs to the field of cell culture devices, in particular to a cell culture bioreactor. Background technique [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, cell bioreactors used for large-scale high-density cultivation of animal cells have become the most important equipment in the biotechnology pharmaceutical industry. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. [0003] Bioreactor refers to equipment that uses living cells or enzymes as biocatalysts to provide a suitable environment for cell proliferation or biochemical reactions. ...

AI Technical Summary

Problems solved by technology

With the continuous development of biotechnology, large-scale animal cell bioreactor culture technology is gradually replacing glass bottle static culture and rotary culture technology, because it can provide the best living environment for the cultured objects in time, so that the most efficient It should be said that this is a qualitative leap in the history of animal cell culture and a major breakthrough in biopharmaceutical technology. It not only simplifies the process, but more importantly, it can produce high-quality products. The current market There are many kinds of bioreactors on the market, including domestic and imported ones, each has its own advantages and disadvantages, and some even have unavoidable flaws, which limit the maximum use of its advantages

[0004] Existing reactors often can only meet the needs of one culture method, and there are many kinds of cell culture methods at present, and different cells have different requirements for culture methods. Moreover, most of the existing reactors are inserted into the tank. The mechanical stirring method, the structure of the stirring device and the problem of microbial contamination caused by its lubricating substances, the incidence of bacteria introduced into the stirring and lubrication is high, once the bacteria are contaminated, it will cause cell death, or due to the shear Cutting causes cell death, and high-density culture often has a low dissolved oxygen level, which cannot meet the needs, and is also an important reason for low density and low expression

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