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Cell culture bioreactor

A bioreactor and cell culture technology, applied in the field of cell culture bioreactors, can solve the problems of large-volume bioreactors, poor ventilation uniformity, cell damage, etc., to reduce the risk of bacterial contamination, increase production, and seal good effect

Pending Publication Date: 2018-08-17
江苏弗洛瑞生物工程设备有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention solves the problem of cell damage caused by air bubbles in existing cell reactors, so as to achieve the effect of increasing cell density, but the uniformity and efficiency of ventilation are poor, and it cannot meet the needs of large-volume bioreactors

Method used

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  • Cell culture bioreactor
  • Cell culture bioreactor
  • Cell culture bioreactor

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Embodiment Construction

[0030] Such as figure 1 As shown, a cell culture bioreactor comprises a tank body 1, an upper air inlet 2 is arranged above the tank body 1, and a lower air inlet 10 is arranged below, and the height of the upper air inlet 2 is higher than that of the tank body 1 The height of the inner liquid level is 50 cm to ensure that the surface air is not directly blown into the cell culture liquid to prevent the generation of more foam.

[0031] Such as figure 1 and figure 2 As shown, a magnetic stirring mechanism 15 is provided in the center of the bottom of the tank body 1, and the magnetic stirring mechanism 15 is connected with the stirring shaft 3 located inside the tank body 1. The top of the stirring shaft 3 is provided with an upper paddle 4, and the stirring shaft 3 is from top to bottom. A first stirring mechanism 5 and a second stirring mechanism distributed alternately are provided in sequence. The first stirring mechanism 5 comprises the fixed ring 51 that is fixed on ...

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Abstract

The invention provides a cell culture bioreactor and belongs to the technical field of cell culture. The cell culture bioreactor comprises a tank body, wherein an upper air inlet is formed in the upper part of the tank body, and a lower air inlet is formed in the lower part of the tank body; a magnetic stirring mechanism is arranged in the bottom center of the tank body; the magnetic stirring mechanism is connected with a stirring shaft in the tank body; an upper blade is arranged at the top end of the stirring shaft; a first stirring mechanism and a second stirring mechanism in alternative distribution are sequentially arranged on the stirring shaft from top to bottom; the second stirring mechanism is longer than the first stirring mechanism; a microbubble ring is arranged below the stirring shaft; the microbubble ring comprises at least two hollow sub-rings with diameter gradually reducing from top to bottom and a first air inlet rod connecting the sub-rings; the first air inlet rodis communicated with the lower air inlet by a second air inlet rod; multiple air holes in uniform distribution are formed in the lower surface of the sub-rings. The cell culture bioreactor provided bythe invention has the advantages of uniform ventilation, high efficiency, uniform mixing of cells and nutrient substances, high yield and uniform product quality while meeting the need for large-volume tank production.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a cell culture bioreactor. Background technique [0002] Bioreactor is the core equipment for large-scale cell suspension culture, which can effectively increase the culture density per unit volume of cells, and is an important pillar for the large-scale production of such products. my country is already the world's largest producer and user of vaccine products, with an annual growth rate of 15%. In recent years, the research and development of antibody drugs and market demand have also increased significantly. In order to improve production efficiency and product quality, effectively reduce production costs, and change the outdated production process of using spinner bottles to culture cells, there is an urgent need for animal cell culture reactors with a production scale of more than 1,000 liters. [0003] The volume of existing cell culture reactors is genera...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/04C12M1/02
CPCC12M27/02C12M29/06
Inventor 虞永胜张剑陈伟
Owner 江苏弗洛瑞生物工程设备有限公司
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