Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell culture biological production device

A production device and cell culture technology, applied in the field of cell culture biological production devices, can solve problems such as low maximum cell density

Inactive Publication Date: 2016-06-01
上海尚优生物科技有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] One of the technical problems to be solved by the present invention is the problem that the maximum cell density achievable in the bioreactor is low when the cell culture bioreactor in the prior art is used for cell culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell culture biological production device
  • Cell culture biological production device
  • Cell culture biological production device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Experimental device

[0050] use Figure 4 The production device shown, where

[0051] 5L bioreactor (working volume 3.5L) and control cabinet: Swiss bio company;

[0052] Aeration tower: self-made

[0053] The body of the aeration tower is a glass tube with an outer diameter of 200mm, a wall thickness of 5mm, and a height of 450mm. The filler is a high borosilicate glass ball with a diameter of 5mm, and the height of the filler layer is 250mm.

[0054] 2. Experimental materials

[0055] Vero cells: from the Shanghai Cell Bank of the Chinese Academy of Sciences;

[0056] Microcarrier cytodexI: American GE Company;

[0057] Basal medium M199 (without NaHCO 3 ): American Hyclone Company;

[0058] M199 basal culture solution: 9.45 parts by weight of the basal medium M199 plus 900 parts by weight of water for injection are dissolved, then the pH is adjusted to 7.20 to 7.30 with a concentration of 5w% sodium bicarbonate aqueous solution, and then diluted to 1000 pa...

Embodiment 2

[0086] 1. Experimental device

[0087] use Figure 5 The production device shown, where

[0088] 5L bioreactor (working volume 3.5L) and control cabinet: Swiss bio company;

[0089] Aeration tower: self-made

[0090] The body of the aeration tower is a glass tube with an outer diameter of 200mm, a wall thickness of 5mm, and a height of 450mm. The filler is a high borosilicate glass ball with a diameter of 5mm, and the height of the filler layer is 250mm.

[0091] Settler: Homemade

[0092] A glass tube with a diameter of 150 mm and a height of 200 mm has a 60° conical bottom at the bottom, and a 15 mm diameter glass tube connected to the tip of the cone bottom.

[0093] 2. Experimental materials

[0094] Vero cells: from the Shanghai Cell Bank of the Chinese Academy of Sciences;

[0095] Microcarrier cytodexI: American GE Company;

[0096] Basal medium M199 (without NaHCO 3 ): American Hyclone Company;

[0097] M199 basal culture solution: 9.45 parts by weight of the ...

Embodiment 3

[0125] 1. Experimental device

[0126] use Image 6 The production device shown, where

[0127] 5L bioreactor (working volume 3.5L) and control cabinet: Swiss bio company;

[0128] Aeration tower: self-made

[0129] The body of the aeration tower is a glass tube with an outer diameter of 200mm, a wall thickness of 5mm, and a height of 450mm. The filler is a high borosilicate glass ball with a diameter of 5mm, and the height of the filler layer is 250mm.

[0130] Settler: Homemade

[0131] A glass tube with a diameter of 150 mm and a height of 200 mm has a 60° conical bottom at the bottom, and a 15 mm diameter glass tube connected to the tip of the cone bottom.

[0132] Cell debris separator: self-made hydrocyclone, the shape is as follows:

[0133] A glass tube with a diameter of 25mm and a glass tube with an inner diameter of 15mm. The upper part is sealed with flat glass, and the inner tube grows out; the inner and outer FRP diameters form a uniform ring; the lower part i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Outer diameteraaaaaaaaaa
Wall thicknessaaaaaaaaaa
Heightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a cell culture biological production device, mainly aiming at solving the problem that when the cell culture biological reaction device in the prior art is used for carrying out cell culture, the maximum cell density in the bioreactor is low. The cell culture biological production device comprises a cell culture bioreactor and a culture solution oxygenation tower, wherein the inside of the oxygenation tower has the following arrangement: the upper space is provided with a liquid distributor, the lower space is provided with an oxygenation tower gas distributor communicated with an oxygenation gas inlet, and a filler layer is arranged between the liquid distributor and the oxygenation tower gas distributor; an oxygenation tower tail gas discharge hole is formed in the top of the oxygenation tower; the production device is provided with an oxygenation tower feeding channel I used for taking a liquid material from the reactor and conveying the liquid material to the top of the oxygenation tower and communicated with the liquid distributor; and the production device is provided with a liquid material back conveying channel II used for taking the liquid material from the bottom of the oxygenation tower and conveying the liquid material to the reactor.

Description

technical field [0001] The present invention relates to a cell culture biological production device. Background technique [0002] Animal cell culture is the basis of cell-derived human vaccines, cell-derived animal vaccines, antibody drugs and some recombinant protein drugs. The number of cells cultured and proliferated per unit volume is the basis for the yield of the above products. Trying to increase the density of animal cell culture and providing more favorable conditions for further product increase has always been the focus of the industrialization and engineering of the above-mentioned products. [0003] Animal cell culture is more anchorage-dependent cell culture, that is, cells must attach to a suitable surface for growth, metabolism and proliferation. [0004] To provide cells with an attachment surface for growth, cells and culture fluid are initially placed in a square bottle, which is laid flat with a larger side down, and the cells grow on this side. The b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M3/02C12M1/34C12M1/04C12M1/02C12N5/07
Inventor 朱文洲朱孜谦
Owner 上海尚优生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com